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3D culture of HepaRG cells in GelMa and its application to bioprinting of a multicellular hepatic model

hal.structure.identifierInstitut de recherche en santé, environnement et travail [Irset]
dc.contributor.authorCUVELLIER, Marie
hal.structure.identifierInstitut de recherche en santé, environnement et travail [Irset]
dc.contributor.authorEZAN, Frédéric
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorOLIVEIRA, Hugo
hal.structure.identifierInstitut de recherche en santé, environnement et travail [Irset]
dc.contributor.authorROSE, Sophie
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorFRICAIN, J.-C.
hal.structure.identifierInstitut de recherche en santé, environnement et travail [Irset]
dc.contributor.authorLANGOUËT, Sophie
hal.structure.identifierInstitut de recherche en santé, environnement et travail [Irset]
dc.contributor.authorLEGAGNEUX, Vincent
hal.structure.identifierInstitut de recherche en santé, environnement et travail [Irset]
dc.contributor.authorBAFFET, Georges
dc.date.accessioned2021-06-10T07:03:25Z
dc.date.available2021-06-10T07:03:25Z
dc.date.issued2020-12-16
dc.identifier.issn0142-9612
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/78914
dc.description.abstractEnBioprinting is an emergent technology that has already demonstrated the capacity to create complex and/or vascularized multicellular structures with defined and organized architectures, in a reproducible and high throughput way. Here, we present the implementation of a complex liver model by the development of a three-dimensional extrusion bioprinting process, including parameters for matrix polymerization of methacrylated gelatin, using two hepatic cell lines, Huh7 and HepaRG. The printed structures exhibited long-term viability (28 days), proliferative ability, a relevant hepatocyte phenotype and functions equivalent to or better than those of their 2D counterparts using standard DMSO treatment. This work served as a basis for the bioprinting of complex multicellular models associating the hepatic parenchymal cells, HepaRG, with stellate cells (LX-2) and endothelial cells (HUVECs), able of colonizing the surface of the structure and thus recreating a pseudo endothelial barrier. When bioprinted in 3D monocultures, LX-2 expression was modulated by TGFβ-1 toward the induction of myofibroblastic genes such as ACTA2 and COL1A1. In 3D multicellular bioprinted structures comprising HepaRG, LX-2 and endothelial cells, we evidenced fibrillar collagen deposition, which is never observed in monocultures of either HepaRG or LX-2 alone. These observations indicate that a precise control of cellular communication is required to recapitulate key steps of fibrogenesis. Bioprinted 3D co-cultures therefore open up new perspectives in studying the molecular and cellular basis of fibrosis development and provide better access to potential inducers and inhibitors of collagen expression and deposition.
dc.language.isoen
dc.publisherElsevier
dc.subject.en3D liver models
dc.subject.enBioprinting
dc.subject.enHepaRG
dc.subject.enHepatocyte
dc.subject.enMethacrylated gelatin
dc.title.en3D culture of HepaRG cells in GelMa and its application to bioprinting of a multicellular hepatic model
dc.typeArticle de revue
dc.identifier.doi10.1016/j.biomaterials.2020.120611
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalBiomaterials
bordeaux.page120611
bordeaux.volume269
bordeaux.hal.laboratoriesBioingénierie Tissulaire (BioTis) - U1026*
bordeaux.institutionCNRS
bordeaux.institutionINSERM
bordeaux.institutionCHU de Bordeaux
bordeaux.institutionInstitut Bergonié
bordeaux.peerReviewedoui
hal.identifierhal-03095742
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03095742v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biomaterials&rft.date=2020-12-16&rft.volume=269&rft.spage=120611&rft.epage=120611&rft.eissn=0142-9612&rft.issn=0142-9612&rft.au=CUVELLIER,%20Marie&EZAN,%20Fr%C3%A9d%C3%A9ric&OLIVEIRA,%20Hugo&ROSE,%20Sophie&FRICAIN,%20J.-C.&rft.genre=article


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