3D culture of HepaRG cells in GelMa and its application to bioprinting of a multicellular hepatic model
| hal.structure.identifier | Institut de recherche en santé, environnement et travail [Irset] | |
| dc.contributor.author | CUVELLIER, Marie | |
| hal.structure.identifier | Institut de recherche en santé, environnement et travail [Irset] | |
| dc.contributor.author | EZAN, Frédéric | |
| hal.structure.identifier | Bioingénierie tissulaire [BIOTIS] | |
| dc.contributor.author | OLIVEIRA, Hugo | |
| hal.structure.identifier | Institut de recherche en santé, environnement et travail [Irset] | |
| dc.contributor.author | ROSE, Sophie | |
| hal.structure.identifier | Bioingénierie tissulaire [BIOTIS] | |
| dc.contributor.author | FRICAIN, J.-C. | |
| hal.structure.identifier | Institut de recherche en santé, environnement et travail [Irset] | |
| dc.contributor.author | LANGOUËT, Sophie | |
| hal.structure.identifier | Institut de recherche en santé, environnement et travail [Irset] | |
| dc.contributor.author | LEGAGNEUX, Vincent | |
| hal.structure.identifier | Institut de recherche en santé, environnement et travail [Irset] | |
| dc.contributor.author | BAFFET, Georges | |
| dc.date.accessioned | 2021-06-10T07:03:25Z | |
| dc.date.available | 2021-06-10T07:03:25Z | |
| dc.date.issued | 2020-12-16 | |
| dc.identifier.issn | 0142-9612 | |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/78914 | |
| dc.description.abstractEn | Bioprinting is an emergent technology that has already demonstrated the capacity to create complex and/or vascularized multicellular structures with defined and organized architectures, in a reproducible and high throughput way. Here, we present the implementation of a complex liver model by the development of a three-dimensional extrusion bioprinting process, including parameters for matrix polymerization of methacrylated gelatin, using two hepatic cell lines, Huh7 and HepaRG. The printed structures exhibited long-term viability (28 days), proliferative ability, a relevant hepatocyte phenotype and functions equivalent to or better than those of their 2D counterparts using standard DMSO treatment. This work served as a basis for the bioprinting of complex multicellular models associating the hepatic parenchymal cells, HepaRG, with stellate cells (LX-2) and endothelial cells (HUVECs), able of colonizing the surface of the structure and thus recreating a pseudo endothelial barrier. When bioprinted in 3D monocultures, LX-2 expression was modulated by TGFβ-1 toward the induction of myofibroblastic genes such as ACTA2 and COL1A1. In 3D multicellular bioprinted structures comprising HepaRG, LX-2 and endothelial cells, we evidenced fibrillar collagen deposition, which is never observed in monocultures of either HepaRG or LX-2 alone. These observations indicate that a precise control of cellular communication is required to recapitulate key steps of fibrogenesis. Bioprinted 3D co-cultures therefore open up new perspectives in studying the molecular and cellular basis of fibrosis development and provide better access to potential inducers and inhibitors of collagen expression and deposition. | |
| dc.language.iso | en | |
| dc.publisher | Elsevier | |
| dc.subject.en | 3D liver models | |
| dc.subject.en | Bioprinting | |
| dc.subject.en | HepaRG | |
| dc.subject.en | Hepatocyte | |
| dc.subject.en | Methacrylated gelatin | |
| dc.title.en | 3D culture of HepaRG cells in GelMa and its application to bioprinting of a multicellular hepatic model | |
| dc.type | Article de revue | |
| dc.identifier.doi | 10.1016/j.biomaterials.2020.120611 | |
| dc.subject.hal | Sciences du Vivant [q-bio] | |
| bordeaux.journal | Biomaterials | |
| bordeaux.page | 120611 | |
| bordeaux.volume | 269 | |
| bordeaux.hal.laboratories | Bioingénierie Tissulaire (BioTis) - U1026 | * |
| bordeaux.institution | CNRS | |
| bordeaux.institution | INSERM | |
| bordeaux.institution | CHU de Bordeaux | |
| bordeaux.institution | Institut Bergonié | |
| bordeaux.peerReviewed | oui | |
| hal.identifier | hal-03095742 | |
| hal.version | 1 | |
| hal.origin.link | https://hal.archives-ouvertes.fr//hal-03095742v1 | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biomaterials&rft.date=2020-12-16&rft.volume=269&rft.spage=120611&rft.epage=120611&rft.eissn=0142-9612&rft.issn=0142-9612&rft.au=CUVELLIER,%20Marie&EZAN,%20Fr%C3%A9d%C3%A9ric&OLIVEIRA,%20Hugo&ROSE,%20Sophie&FRICAIN,%20J.-C.&rft.genre=article |
Files in this item
| Files | Size | Format | View |
|---|---|---|---|
|
There are no files associated with this item. |
|||