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Phospholipid Content of Pseudomonas aeruginosa PAO1 Is Modulated by the Growth Phase Rather Than the Immobilization State

dc.rights.licenseopenen_US
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorLE SENECHAL, Caroline
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorCROUZET, Marc
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorCOSTAGLIOLI, Patricia
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorBARTHE, Christophe
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorBURE, Corinne
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorVILAIN, Sebastien
IDREF: 076188744
dc.date.accessioned2020-05-13T13:31:33Z
dc.date.available2020-05-13T13:31:33Z
dc.date.issued2019
dc.identifier.issn0024-4201en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/7562
dc.description.abstractEnBiofilms have significance in medical, industrial, and environmental settings, and can cause important damage. As biofilms are tolerant to various stresses, including antibiotics, it is necessary to better understand their formation. For this reason, we characterized the phospholipidome of Pseudomonas aeruginosa, an opportunistic pathogen involved in numerous infections, during the first steps of the biofilm development. By a liquid chromatography-tandem mass spectrometry time-course analysis over a 24-h period, we compared the phospholipid (PL) composition of immobilized (attached) and planktonic (unattached) P. aeruginosa PAO1 cells. Our results showed that the PL content of P. aeruginosa PAO1 was mainly modulated by the incubation time, thus related to bacterial growth but also, more modestly, by the immobilization state. We observed that relative amounts of PL varied over time with two main profiles and that these profiles are correlated to its fatty acid composition, including the degree of unsaturation. A statistical analysis revealed that the PL contents of both attached and unattached PAO1 cells were significantly different mainly after 3 and 6h of incubation and that the amounts of two PL presented a statistical difference between attached and unattached cells all along the 24-h period: PtdEtn 16:0_18:1 and PtdEtn 18:1_18:1.
dc.language.isoENen_US
dc.subject.enAttachment
dc.subject.enBiofilm
dc.subject.enLipidomics
dc.subject.enMass spectrometry
dc.subject.enPhospholipids
dc.subject.enPseudomonas aeruginosa
dc.title.enPhospholipid Content of Pseudomonas aeruginosa PAO1 Is Modulated by the Growth Phase Rather Than the Immobilization State
dc.typeArticle de revueen_US
dc.identifier.doi10.1002/lipd.12184en_US
dc.subject.halChimie/Matériauxen_US
bordeaux.journalLipidsen_US
bordeaux.page519-529en_US
bordeaux.volume54en_US
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248en_US
bordeaux.issue9en_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-02572243
hal.version1
hal.date.transferred2020-05-13T13:31:53Z
hal.exporttrue
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