Study of single or mixture 1 of tethered peptide on surfaces to promote hMSCs differentiation toward osteoblastic lineage
| dc.rights.license | open | en_US |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | PADIOLLEAU, L. | |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | CHANSEAU, Christel | |
| hal.structure.identifier | Acides Nucléiques : Régulations Naturelle et Artificielle [ARNA] | |
| dc.contributor.author | DURRIEU, S. | |
| dc.contributor.author | CHEVALLIER, P. | |
| dc.contributor.author | LAROCHE, G. | |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | DURRIEU, Marie‐Christine | |
| dc.date.accessioned | 2020-04-10T08:41:18Z | |
| dc.date.available | 2020-04-10T08:41:18Z | |
| dc.date.issued | 2018 | |
| dc.identifier.issn | 2576-6422 | en_US |
| dc.identifier.other | 10.1021/acsabm.8b00236 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/4207 | |
| dc.description.abstractEn | The commitment and differentiation of human mesenchymal stem cells (hMSCs) are guided by bioactive molecules within the extracellular matrix. Among the various approaches to design biomaterials, the functionalization of biomaterial surfaces with peptides from the sequence of proteins from the extracellular matrix is quite common. The purpose of this functionalization is to recruit hMSCs and promote their differentiation into the appropriate lineage. The aim of this work was to investigate the influence of RGD and FHRRIKA peptides and peptide sequences taken from bone morphogenic protein (BMP-2) and histone H4 (osteogenic growth peptide; OGP) either tethered alone or as a mixture on the surface of a model material and to also examine the level of hMSC osteogenic commitment without using a differentiation medium. Grafting of the different peptides was assessed by X-ray photoelectron spectroscopy (XPS), while their surface density was quantified by fluorescence microscopy, and their surface properties were assessed by atomic force microscopy (AFM) and contact angle (CA). The osteogenic commitment of hMSCs cultured on the different surfaces was characterized by immunohistochemistry using Runx-2 as an earlier osteogenic marker and OPN, a late osteogenic marker, and by RT-qPCR through the expression of ColI-a1, Runx-2, and ALP. Biological results show that the osteogenic commitment of the hMSCs was increased on surfaces tethered with a mixture of peptides. Results indicate that tethered peptides in the range of pmol mm–2 were indeed effective in inducing a cellular response after 2 weeks of cell culture without using an osteogenic media. These findings contribute to the research efforts to design biomimetic materials able to induce a response in human stem cells through tethered bioactive molecules for bone tissue engineering. | |
| dc.language.iso | EN | en_US |
| dc.subject.en | stem cells | |
| dc.subject.en | biomimetic materials | |
| dc.subject.en | bone tissue engineering | |
| dc.subject.en | mimetic peptides | |
| dc.subject.en | surface modification | |
| dc.title.en | Study of single or mixture 1 of tethered peptide on surfaces to promote hMSCs differentiation toward osteoblastic lineage | |
| dc.title.alternative | ACS Appl. Bio Mater. | en_US |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1021/acsabm.8b00236 | en_US |
| dc.subject.hal | Chimie/Matériaux | en_US |
| bordeaux.journal | ACS Applied Bio Materials | en_US |
| bordeaux.page | 1800-1809 | en_US |
| bordeaux.volume | 1 | en_US |
| bordeaux.hal.laboratories | Institut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248 | |
| bordeaux.issue | 6 | en_US |
| bordeaux.institution | Bordeaux INP | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| hal.identifier | hal-02539516 | |
| hal.version | 1 | |
| hal.date.transferred | 2020-04-10T08:41:22Z | |
| hal.export | true | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=ACS%20Applied%20Bio%20Materials&rft.date=2018&rft.volume=1&rft.issue=6&rft.spage=1800-1809&rft.epage=1800-1809&rft.eissn=2576-6422&rft.issn=2576-6422&rft.au=PADIOLLEAU,%20L.&CHANSEAU,%20Christel&DURRIEU,%20S.&CHEVALLIER,%20P.&LAROCHE,%20G.&rft.genre=article |
Files in this item
| Files | Size | Format | View |
|---|---|---|---|
|
There are no files associated with this item. |
|||