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A critical assessment of transmethylation procedures for n-3 long-chain polyunsaturated fatty acid quantification of lipid classes

dc.rights.licenseopenen_US
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hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
hal.structure.identifierITERG
dc.contributor.authorSEHL, Anthony
hal.structure.identifierITERG
dc.contributor.authorCOUEDELO, Leslie
hal.structure.identifierITERG
dc.contributor.authorFONSECA, Laurence
hal.structure.identifierITERG
dc.contributor.authorVAYSSE, Carole
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorCANSELL, Maud
dc.date.accessioned2020-04-08T09:12:23Z
dc.date.available2020-04-08T09:12:23Z
dc.date.issued2018
dc.identifier.issn0308-8146en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/4161
dc.description.abstractEnLipid transmethylation methods described in the literature are not always evaluated with care so to insure that the methods are effective, especially on food matrix or biological samples containing polyunsaturated fatty acid (PUFA). The aim of the present study was to select a method suitable for all lipid species rich in long chain n-3 PUFA. Three published methods were adapted and applied on individual lipid classes. Lipid (trans)methylation efficiency was characterized in terms of reaction yield and gas chromatography (GC) analysis. The acid-catalyzed method was unable to convert triglycerides and sterol esters, while the method using an incubation at a moderate temperature was ineffective on phospholipids and sterol esters. On the whole only the method using sodium methoxide and sulfuric acid was effective on lipid classes taken individually or in a complex medium. This study highlighted the use of an appropriate (trans)methylation method for insuring an accurate fatty acid composition.
dc.language.isoENen_US
dc.subject.enMethylation
dc.subject.enFatty acid methyl esters
dc.subject.enPolyunsaturated fatty acids
dc.subject.enGas chromatography
dc.subject.enHigh-performance thin layer chromatography
dc.title.enA critical assessment of transmethylation procedures for n-3 long-chain polyunsaturated fatty acid quantification of lipid classes
dc.typeArticle de revueen_US
dc.identifier.doi10.1016/j.foodchem.2018.01.060en_US
dc.subject.halChimie/Matériauxen_US
bordeaux.journalFood Chemistryen_US
bordeaux.page1-8en_US
bordeaux.volume251en_US
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-02536529
hal.version1
hal.date.transferred2020-04-08T09:12:28Z
hal.exporttrue
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