SEHL, Anthony; COUEDELO, Leslie; FONSECA, Laurence; VAYSSE, Carole; CANSELL, Maud

doi:10.1016/j.foodchem.2018.01.060

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SEHL, Anthony
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]
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COUEDELO, Leslie
ITERG

FONSECA, Laurence
ITERG

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Food Chemistry. 2018, vol. 251, p. 1-8

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Lipid transmethylation methods described in the literature are not always evaluated with care so to insure that the methods are effective, especially on food matrix or biological samples containing polyunsaturated fatty acid (PUFA). The aim of the present study was to select a method suitable for all lipid species rich in long chain n-3 PUFA. Three published methods were adapted and applied on individual lipid classes. Lipid (trans)methylation efficiency was characterized in terms of reaction yield and gas chromatography (GC) analysis. The acid-catalyzed method was unable to convert triglycerides and sterol esters, while the method using an incubation at a moderate temperature was ineffective on phospholipids and sterol esters. On the whole only the method using sodium methoxide and sulfuric acid was effective on lipid classes taken individually or in a complex medium. This study highlighted the use of an appropriate (trans)methylation method for insuring an accurate fatty acid composition.< Leer menos

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Methylation

Fatty acid methyl esters

Polyunsaturated fatty acids

Gas chromatography

High-performance thin layer chromatography

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A critical assessment of transmethylation procedures for n-3 long-chain polyunsaturated fatty acid quantification of lipid classes

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