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A genetic network for systemic RNA silencing in plants

dc.rights.licenseopenen_US
dc.contributor.authorCHEN, Weiwei
dc.contributor.authorZHANG, Xian
dc.contributor.authorFAN, Yaya
dc.contributor.authorLI, Bin
dc.contributor.authorRYABOV, Eugene
dc.contributor.authorSHI, Nongnong
dc.contributor.authorZHAO, Mei
dc.contributor.authorYU, Zhiming
dc.contributor.authorQIN, Cheng
dc.contributor.authorZHENG, Qianqian
dc.contributor.authorZHANG, Pengcheng
dc.contributor.authorWANG, Huizhong
dc.contributor.authorJACKSON, Stephen
dc.contributor.authorCHENG, Qi
dc.contributor.authorLIU, Yule
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorGALLUSCI, Philippe
dc.contributor.authorHONG, Yiguo
dc.date.accessioned2020-04-07T08:34:25Z
dc.date.available2020-04-07T08:34:25Z
dc.date.issued2018
dc.identifier.issn0032-0889en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/4132
dc.description.abstractEnNon-cell autonomous RNA silencing can spread from cell to cell and over long distances in animals and plants. However, the genetic requirements and signals involved in plant mobile gene silencing are poorly understood. Here, we identified a DICERLIKE2 (DCL2)-dependent mechanism for systemic spread of posttranscriptional RNA silencing, also known as posttranscriptional gene silencing (PTGS), in Nicotiana benthamiana. Using a suite of transgenic DCL RNAi lines coupled with a GFP reporter, we demonstrated that N. benthamiana DCL1, DCL2, DCL3, and DCL4 are required to produce microRNAs and 22, 24, and 21nt small interfering RNAs (siRNAs), respectively. All investigated siRNAs produced in local incipient cells were present at low levels in distal tissues. Inhibition of DCL2 expression reduced the spread of gene silencing, while suppression of DCL3 or DCL4 expression enhanced systemic PTGS. In contrast to DCL4 RNAi lines, DCL2-DCL4 double-RNAi lines developed systemic PTGS similar to that observed in DCL2 RNAi. We further showed that the 21 or 24 nt local siRNAs produced by DCL4 or DCL3 were not involved in long-distance gene silencing. Grafting experiments demonstrated that DCL2 was required in the scion to respond to the signal, but not in the rootstock to produce/send the signal. These results suggest a coordinated DCL genetic pathway in which DCL2 plays an essential role in systemic PTGS in N. benthamiana, while both DCL4 and DCL3 attenuate systemic PTGS. We discuss the potential role of 21, 22, and 24 nt siRNAs in systemic PTGS.
dc.language.isoENen_US
dc.subjectNicotiana Benthamiana
dc.subjectInteraction porte greffe Scion
dc.subjectExpression des gènes
dc.subjectGreffage
dc.subjectApproche systémique
dc.title.enA genetic network for systemic RNA silencing in plants
dc.title.alternativePlant physiol.en_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1104/pp.17.01828en_US
dc.subject.halSciences du Vivant [q-bio]/Biologie végétaleen_US
dc.identifier.pubmed29439213en_US
bordeaux.journalPlant Physiologyen_US
bordeaux.page2700-2719en_US
bordeaux.volume176en_US
bordeaux.hal.laboratoriesEcophysiologie et Génomique Fonctionnelle de la Vigne (EGFV) - UMR 1287en_US
bordeaux.issue4en_US
bordeaux.institutionBordeaux Sciences Agroen_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-02534590
hal.version1
hal.date.transferred2020-04-07T08:34:33Z
hal.exporttrue
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