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The Xylella fastidosa RTX operons: evidence for the evolution of protein mosaics through novel genetic exchanges

dc.rights.licenseopenen_US
dc.contributor.authorMATTHEWS, Mark A.
dc.contributor.authorSYVANEN, Michael
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorGAMBETTA, Gregory
ORCID: 0000-0002-8838-5050
IDREF: 225449641
dc.date.accessioned2020-04-06T10:20:00Z
dc.date.available2020-04-06T10:20:00Z
dc.date.issued2018
dc.identifier.issn1471-2164en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/4118
dc.description.abstractEnBackground: Xylella fastidiosa (Xf) is a gram negative bacterium inhabiting the plant vascular system. In most species this bacterium lives as a benign symbiote, but in several agriculturally important plants (e.g. coffee, citrus, grapevine) Xf is pathogenic. Xf has four loci encoding homologues to hemolysin RTX proteins, virulence factors involved in a wide range of plant pathogen interactions. [br/] Results: We show that all four genes are expressed during pathogenesis in grapevine. The sequences from these four genes have a complex repetitive structure. At the C-termini, sequence diversity between strains is what would be expected from orthologous genes. However, within strains there is no N-terminal homology, indicating these loci encode RTXs of different functions and/or specificities. More striking is that many of the orthologous loci between strains share this extreme variation at the N-termini. Thus these RTX orthologues are most easily visualized as fusions between the orthologous C-termini and different N-termini. Further, the four genes are found in operons having a peculiar structure with an extensively duplicated module encoding a small protein with homology to the N-terminal region of the full length RTX. Surprisingly, some of these small peptides are most similar not to their corresponding full length RTX, but to the N-termini of RTXs from other Xf strains, and even other remotely related species. [br/] Conclusions: These results demonstrate that these genes are expressed in planta during pathogenesis. Their structure suggests extensive evolutionary restructuring through horizontal gene transfers and heterologous recombination mechanisms. The sum of the evidence suggests these repetitive modules are a novel kind of mobile genetic element.
dc.language.isoENen_US
dc.subjectXylella Fastidiosa
dc.subjectVitis Vinifera
dc.subjectHémolysine
dc.subjectFlux de gènes
dc.subjectPathogénèse
dc.subjectExpression des gènes
dc.subjectMaladie de Pierce
dc.subjectPcr
dc.subject.enHemolysin
dc.subject.enHorizontal Gene Transfer
dc.subject.enLateral Gene Transfer
dc.subject.enOrphan
dc.subject.enPierce’s Disease
dc.title.enThe Xylella fastidosa RTX operons: evidence for the evolution of protein mosaics through novel genetic exchanges
dc.typeArticle de revueen_US
dc.identifier.doi10.1186/s12864-018-4731-9en_US
dc.subject.halSciences du Vivant [q-bio]/Biologie végétaleen_US
dc.identifier.pubmed29728072en_US
bordeaux.journalBMC Genomicsen_US
bordeaux.page1-11en_US
bordeaux.volume19en_US
bordeaux.hal.laboratoriesEcophysiologie et Génomique Fonctionnelle de la Vigne (EGFV) - UMR 1287en_US
bordeaux.issue1en_US
bordeaux.institutionBordeaux Sciences Agroen_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-02533258
hal.version1
hal.date.transferred2020-04-06T10:20:07Z
hal.exporttrue
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