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dc.rights.licenseopenen_US
dc.contributor.authorHOW-KIT, Alexandre
dc.contributor.authorTEYSSIER, Emeline
dc.contributor.authorDELEUZE, Jean-François
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorGALLUSCI, Philippe
dc.date.accessioned2020-04-01T14:47:05Z
dc.date.available2020-04-01T14:47:05Z
dc.date.issued2017
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/4059
dc.description.abstractEnIn contrast to mammals where DNA methylation occurs near exclusively at CG dinucleotides, all cytosines of plant genomes can be methylated irrespective to the sequence context, including the symmetrical CG and CHG sequence contexts, and the nonsymmetrical CHH sequence context. Plant genomes do not present CG islands as found in mammalian genomes where a high frequency of CG can be observed at some promoter regions. So far, the methylome of several plants has been described showing variations of both methylation levels between plants, which ranged from 5% in Arabidopsis thaliana to more than 30% in corn, but also of the proportion of methylated cytosines in the CG, CHG, and CHH sequence contexts. DNA methylation was shown to have important roles in the development of many organisms including plants. In this later case, DNA methylation is involved in the regulation of flowering time, flower sex determination, seed development, or fruit ripening. To date, a wide range of low to high resolution methods initially developed for animal genomes allow the analysis of DNA methylation at specific loci or globally of whole genomes. Indeed, part of these methods is not applicable to plants due to the specificities of their methylome; however, some have been successfully modified to overcome the complexity of plant DNA methylation. In this chapter, we describe an exhaustive list of methods devoted to the locus-specific analysis of DNA methylation which have proven to be applicable to plant genomes and their potential use in high throughput screening of plant population.
dc.language.isoENen_US
dc.publisherSpringer International Publishing AG, Cham(CHE)en_US
dc.source.titlePlant Epigeneticsen_US
dc.subjectMéthylation
dc.subjectDinucléotide
dc.subjectCytosine
dc.subjectArabidopsis thaliana
dc.subjectMais cru
dc.subjectPyroséquençage
dc.subject.enPlant Dna Methylation
dc.subject.enDna Methylation Analysis Methods
dc.subject.enPyrosequencing
dc.subject.enLocus-Specific Methods
dc.title.enLocus-specific DNA methylation analysis and applications to plants
dc.title.alternativeRNA technol.en_US
dc.typeChapitre d'ouvrageen_US
dc.identifier.doi10.1007/978-3-319-55520-1_16en_US
dc.subject.halSciences du Vivant [q-bio]/Biologie végétaleen_US
bordeaux.page303-327en_US
bordeaux.hal.laboratoriesEcophysiologie et Génomique Fonctionnelle de la Vigne (EGFV) - UMR 1287en_US
bordeaux.institutionBordeaux Sciences Agroen_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.inpressnonen_US
hal.identifierhal-02528179
hal.version1
hal.date.transferred2020-04-01T14:47:15Z
hal.exporttrue
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