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Characterization, functional validation and gene expression patterns of two 14-3-3 isoforms from Vitis vinifera

dc.rights.licenseopenen_US
dc.contributor.authorFARACE, Giovanni
dc.contributor.authorVISCONTI, S.
dc.contributor.authorMARCHIVE, Chloé
dc.contributor.authorADUCCI, P.
dc.contributor.authorMARRA, M.
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorTROSSAT-MAGNIN, Claudine
IDREF: 225347954
dc.date.accessioned2020-03-18T17:54:50Z
dc.date.available2020-03-18T17:54:50Z
dc.date.issued2015
dc.identifier.issn0042-7500en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/3849
dc.description.abstractEnIn eukaryotic cells, 14-3-3 proteins constitute a family of conserved regulatory proteins which bind to a large number of phosphorylated partners. Although a great body of evidences accumulated in herbaceous species indicates that 14-3-3s play key roles in regulation of plant growth, information about function in tree plants is still very scarce. In this paper, we report the characterization of two novel 14-3-3s from grapevine (Vitis vinifera 'Cabernet Sauvignon') named VV1_CS and VV2_CS. The VV1_CS and VV2_CS cDNA were expressed in Escherichia coli and the recombinant proteins were shown to be functional proteins using plasma membrane H+-ATPase as target. Transcript analysis during grape development or under elicitor-induced stress revealed differential expression of the two 14-3-3 isoforms.
dc.language.isoFRen_US
dc.subjectVitis Vinifera
dc.subjectCellule Eucaryote
dc.subjectEscherichia Coli
dc.subjectCabernet Sauvignon
dc.subjectSaccharomyces Cerevisiae
dc.subjectExpression Des Gènes
dc.subjectPcr
dc.subjectIsoforme
dc.subject.en14-3-3 Proteins
dc.subject.enVitis Vinifera
dc.title.enCharacterization, functional validation and gene expression patterns of two 14-3-3 isoforms from Vitis vinifera
dc.typeArticle de revueen_US
dc.subject.halSciences du Vivant [q-bio]/Biologie végétaleen_US
bordeaux.journalVitisen_US
bordeaux.page33-40en_US
bordeaux.volume54en_US
bordeaux.hal.laboratoriesEcophysiologie et Génomique Fonctionnelle de la Vigne (EGFV) - UMR 1287en_US
bordeaux.issue1en_US
bordeaux.institutionBordeaux Sciences Agroen_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-02511492
hal.version1
hal.date.transferred2020-03-18T17:54:52Z
hal.exporttrue
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