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G-quadruplexes are promoter elements controlling nucleosome exclusion and RNA polymerase II pausing.

dc.rights.licenseopenen_US
dc.contributor.authorESNAULT, Cyril
dc.contributor.authorZINE EL AABIDINE, Amal
dc.contributor.authorROBERT, Marie-Cécile
dc.contributor.authorCUCCHIARINI, Anne
dc.contributor.authorMAGAT, Talha
dc.contributor.authorPIGEOT, Alexia
dc.contributor.authorBOUCHOUIKA, Soumya
dc.contributor.authorGARCIA-OLIVER, Encar
dc.contributor.authorGAWRON, Kevin
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorBASYUK, Eugénia
dc.contributor.authorKARPINSKA, Magdalena A
dc.contributor.authorKOZULIC-PIRHER, Alja
dc.contributor.authorLUO, Yu
dc.contributor.authorVERGA, Daniela
dc.contributor.authorMOURAD, Raphael
dc.contributor.authorRADULESCU, Ovidiu
dc.contributor.authorMERGNY, Jean-Louis
dc.contributor.authorBERTRAND, Edouard
dc.contributor.authorANDRAU, Jean-Christophe
dc.date.accessioned2025-09-01T08:17:21Z
dc.date.available2025-09-01T08:17:21Z
dc.date.issued2025-08-01
dc.identifier.issn1546-1718en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/207544
dc.description.abstractEnDespite their central role in transcription, it has been difficult to define universal sequences associated with eukaryotic promoters. Within the chromatin context, recruitment of transcriptional machinery requires promoter opening, but how DNA elements contribute to this process is unclear. Here we show that G-quadruplex (G4) secondary DNA structures are highly enriched at mammalian promoters. G4s are located at the deepest point of nucleosome exclusion at promoters and correlate with maximum promoter activity. We found that experimental G4s exclude nucleosomes in vivo and in vitro while favouring strong positioning. At model promoters, impairing G4s affected both transcriptional activity and chromatin opening. G4 destabilization also resulted in an inactive promoter state and affected the transition to effective RNA production. Finally, G4 stabilization resulted in global reduction of proximal promoter pausing. Altogether, our data introduce G4s as bona fide promoter elements allowing nucleosome exclusion and facilitating pause-release by RNA polymerase II.
dc.language.isoENen_US
dc.subject.enNucleosomes
dc.subject.enPromoter Regions
dc.subject.enGenetic
dc.subject.enRNA Polymerase II
dc.subject.enG-Quadruplexes
dc.subject.enHumans
dc.subject.enTranscription
dc.subject.enGenetic
dc.subject.enAnimals
dc.subject.enChromatin
dc.subject.enMice
dc.subject.enDNA
dc.title.enG-quadruplexes are promoter elements controlling nucleosome exclusion and RNA polymerase II pausing.
dc.title.alternativeNat Geneten_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1038/s41588-025-02263-6en_US
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologieen_US
dc.identifier.pubmed40696173en_US
bordeaux.journalNature Geneticsen_US
bordeaux.page1981-1993en_US
bordeaux.volume57en_US
bordeaux.hal.laboratoriesMFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234en_US
bordeaux.issue8en_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcepubmed
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hal.popularnonen_US
hal.audienceInternationaleen_US
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dc.rights.ccPas de Licence CCen_US
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