Chemical exchange saturation transfer magnetic resonance imaging for metabolic mapping of ripening tomato fruit: Quite a challenge!
DEBORDE, Catherine
Unité de recherche sur les Biopolymères, Interactions Assemblages [BIA]
BIBS - Plateforme Bioressources : Imagerie, Biochimie & Structure
INRAE, PROBE Research Infrastructure
Biologie du fruit et pathologie [BFP]
MetaboHUB-Bordeaux
Unité de recherche sur les Biopolymères, Interactions Assemblages [BIA]
BIBS - Plateforme Bioressources : Imagerie, Biochimie & Structure
INRAE, PROBE Research Infrastructure
Biologie du fruit et pathologie [BFP]
MetaboHUB-Bordeaux
PAGÈS, Guilhem
Qualité des Produits Animaux [QuaPA]
INRAE, PROBE Research Infrastructure
MetaboHUB-Bordeaux
Leer más >
Qualité des Produits Animaux [QuaPA]
INRAE, PROBE Research Infrastructure
MetaboHUB-Bordeaux
DEBORDE, Catherine
Unité de recherche sur les Biopolymères, Interactions Assemblages [BIA]
BIBS - Plateforme Bioressources : Imagerie, Biochimie & Structure
INRAE, PROBE Research Infrastructure
Biologie du fruit et pathologie [BFP]
MetaboHUB-Bordeaux
Unité de recherche sur les Biopolymères, Interactions Assemblages [BIA]
BIBS - Plateforme Bioressources : Imagerie, Biochimie & Structure
INRAE, PROBE Research Infrastructure
Biologie du fruit et pathologie [BFP]
MetaboHUB-Bordeaux
PAGÈS, Guilhem
Qualité des Produits Animaux [QuaPA]
INRAE, PROBE Research Infrastructure
MetaboHUB-Bordeaux
< Leer menos
Qualité des Produits Animaux [QuaPA]
INRAE, PROBE Research Infrastructure
MetaboHUB-Bordeaux
Idioma
en
Article de revue
Este ítem está publicado en
Food Chemistry. 2025-07, vol. 493, n° 1, p. 145630
Elsevier
Resumen en inglés
Chemical Exchange Saturation Transfer Magnetic Resonance Imaging (CEST-MRI) is an established tool in animal or human MRI to indirectly visualize metabolites in vivo. We used CEST-MRI to map the major metabolites in tomato ...Leer más >
Chemical Exchange Saturation Transfer Magnetic Resonance Imaging (CEST-MRI) is an established tool in animal or human MRI to indirectly visualize metabolites in vivo. We used CEST-MRI to map the major metabolites in tomato fruit at tissue level throughout ripening. Phantom experiments showed a CEST effect in the 1 and 3 ppm chemical shifts for major soluble sugars and free amino acids, respectively, and a tendency for the CEST signal to decrease from pH 4.7 to 3.7. Fruit images for hydroxyl or amine moieties were well-contrasted for all ripening stages, showing gradients within and between tissues. Mean hydroxyl-CEST signals correlated with the ex vivo concentrations of soluble sugars of dissected tissues, although this was not the case for amino acids. This approach holds much promise for monitoring composition changes in vivo within a plant organ tissue.< Leer menos
Palabras clave en inglés
NMR
MRI
Fruit development and ripening
Tomato
Proyecto ANR
Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation - ANR-11-INBS-0010
Orígen
Importado de HalCentros de investigación