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Evaluation of DNA damage and cytotoxicity of polyurethane-based nano- and microparticles as promising biomaterials for drug delivery systems

dc.rights.licenseopen
hal.structure.identifierLab Virol Aplicada [UFSC]
dc.contributor.authorCAON, Thiago
hal.structure.identifierLab Estudos Mat Polimer [UFSC]
dc.contributor.authorZANETTI-RAMOS, Betina Giehl
hal.structure.identifierDept Ciencias Farmaceut [UFSC]
dc.contributor.authorLEMOS-SENNA, Elenara
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 4 LCPO : Polymer Materials for Electronic, Energy, Information and Communication Technologies
dc.contributor.authorCLOUTET, Eric
IDREF: 151048681
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 2 LCPO : Biopolymers & Bio-sourced Polymers
dc.contributor.authorCRAMAIL, Henri
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
dc.contributor.authorBORSALI, Redouane
hal.structure.identifierLab Estudos Mat Polimer [UFSC]
dc.contributor.authorSOLDI, Valdir
hal.structure.identifierLab Virol Aplicada [UFSC]
dc.contributor.authorSIMOES, Claudia Maria Oliveira
dc.date.accessioned2020
dc.date.available2020
dc.date.issued2010
dc.identifier.issn1388-0764
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/20587
dc.description.abstractEnThe in vitro cytotoxicity and DNA damage evaluation of biodegradable polyurethane-based micro- and nanoparticles were carried out on animal fibroblasts. For cytotoxicity measurement and primary DNA damage evaluation, MTT and Comet assays were used, respectively. Different formulations were tested to evaluate the influence of chemical composition and physicochemical characteristics of particles on cell toxicity. No inhibition of cells growth surrounding the polyurethane particles was observed. On the other hand, a decrease of cell viability was verified when the anionic surfactant sodium dodecyl sulfate (SDS) was used as droplets stabilizer of monomeric phase. Polyurethane nanoparticles stabilized with Tween 80 and Pluronic F68 caused minor cytotoxic effects. These results indicated that the surface charge plays an important role on cytotoxicity. Particles synthesized from MDI displayed a higher cytotoxicity than those synthesized from IPDI. Size and physicochemical properties of the particles may explain the higher degree of DNA damage produced by two tested formulations. In this way, a rational choice of particles' constituents based on their cytotoxicity and genotoxicity could be very useful for conceiving biomaterials to be used as drug delivering systems.
dc.language.isoen
dc.publisherSpringer Verlag
dc.subject.enDNA damage
dc.subject.enNanomedicine
dc.subject.enMTT assay
dc.subject.enComet assay
dc.subject.enPolyurethane particles
dc.subject.enSafety
dc.subject.enCytotoxicity
dc.title.enEvaluation of DNA damage and cytotoxicity of polyurethane-based nano- and microparticles as promising biomaterials for drug delivery systems
dc.typeArticle de revue
dc.identifier.doi10.1007/s11051-009-9828-2
dc.subject.halChimie/Polymères
bordeaux.journalJournal of Nanoparticle Research
bordeaux.page1655-1665
bordeaux.volume12
bordeaux.hal.laboratoriesLaboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629*
bordeaux.issue5
bordeaux.institutionBordeaux INP
bordeaux.institutionUniversité de Bordeaux
bordeaux.peerReviewedoui
hal.identifierhal-00503191
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00503191v1
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