Sensory neurons from dorsal root ganglia regulate endothelial cell function in extracellular matrix remodelling.
| dc.rights.license | open | en_US |
| hal.structure.identifier | Bioingénierie tissulaire [BIOTIS] | |
| dc.contributor.author | LEROUX, Alice | |
| hal.structure.identifier | Bioingénierie tissulaire [BIOTIS] | |
| dc.contributor.author | PAIVA DOS SANTOS, Bruno | |
| hal.structure.identifier | Laboratoire du Futur [LOF] | |
| dc.contributor.author | LENG, Jacques | |
| hal.structure.identifier | Bioingénierie tissulaire [BIOTIS] | |
| dc.contributor.author | OLIVEIRA, Hugo | |
| hal.structure.identifier | Bioingénierie tissulaire [BIOTIS] | |
| dc.contributor.author | AMEDEE, Joelle
ORCID: 0000-0002-4888-0129 IDREF: 87485605 | |
| dc.date.accessioned | 2025-01-23T17:45:13Z | |
| dc.date.available | 2025-01-23T17:45:13Z | |
| dc.date.issued | 2020-10-19 | |
| dc.identifier.issn | 1478-811X | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/204531 | |
| dc.description.abstractEn | Recent physiological and experimental data highlight the role of the sensory nervous system in bone repair, but its precise role on angiogenesis in a bone regeneration context is still unknown. Our previous work demonstrated that sensory neurons (SNs) induce the osteoblastic differentiation of mesenchymal stem cells, but the influence of SNs on endothelial cells (ECs) was not studied. Here, in order to study in vitro the interplay between SNs and ECs, we used microfluidic devices as an indirect co-culture model. Gene expression analysis of angiogenic markers, as well as measurements of metalloproteinases protein levels and enzymatic activity, were performed. We were able to demonstrate that two sensory neuropeptides, calcitonin gene-related peptide (CGRP) and substance P (SP), were involved in the transcriptional upregulation of angiogenic markers (vascular endothelial growth factor, angiopoietin 1, type 4 collagen, matrix metalloproteinase 2) in ECs. Co-cultures of ECs with SNs also increased the protein level and enzymatic activity of matrix metalloproteinases 2 and 9 (MMP2/MMP9) in ECs. Our results suggest a role of sensory neurons, and more specifically of CGRP and SP, in the remodelling of endothelial cells extracellular matrix, thus supporting and enhancing the angiogenesis process. Video abstract. | |
| dc.language.iso | EN | en_US |
| dc.rights | Attribution 3.0 United States | * |
| dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | * |
| dc.subject.en | Animals | |
| dc.subject.en | Calcitonin Gene-Related Peptide | |
| dc.subject.en | Endothelial Cells | |
| dc.subject.en | Extracellular Matrix | |
| dc.subject.en | Female | |
| dc.subject.en | Ganglia | |
| dc.subject.en | Spinal | |
| dc.subject.en | Gene Expression Regulation | |
| dc.subject.en | Matrix Metalloproteinases | |
| dc.subject.en | Microfluidics | |
| dc.subject.en | Models | |
| dc.subject.en | Biological | |
| dc.subject.en | Neurites | |
| dc.subject.en | Osteogenesis | |
| dc.subject.en | Rats | |
| dc.subject.en | Wistar | |
| dc.subject.en | Sensory Receptor Cells | |
| dc.subject.en | Substance P | |
| dc.title.en | Sensory neurons from dorsal root ganglia regulate endothelial cell function in extracellular matrix remodelling. | |
| dc.title.alternative | Cell Commun Signal | en_US |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1186/s12964-020-00656-0 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio] | en_US |
| dc.identifier.pubmed | 33076927 | en_US |
| bordeaux.journal | Cell Communication and Signaling | en_US |
| bordeaux.page | 162 | en_US |
| bordeaux.volume | 18 | en_US |
| bordeaux.hal.laboratories | Bioingénierie Tissulaire (BioTis) - U1026 | en_US |
| bordeaux.issue | 1 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.institution | INSERM | en_US |
| bordeaux.institution | CHU de Bordeaux | en_US |
| bordeaux.institution | Institut Bergonié | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.identifier.funderID | Agence de l'innovation de Défense | en_US |
| bordeaux.identifier.funderID | Fondation pour la Recherche Médicale | en_US |
| bordeaux.identifier.funderID | Conseil Régional Aquitaine | en_US |
| bordeaux.import.source | pubmed | |
| hal.popular | non | en_US |
| hal.audience | Internationale | en_US |
| hal.export | false | |
| workflow.import.source | pubmed | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Cell%20Communication%20and%20Signaling&rft.date=2020-10-19&rft.volume=18&rft.issue=1&rft.spage=162&rft.epage=162&rft.eissn=1478-811X&rft.issn=1478-811X&rft.au=LEROUX,%20Alice&PAIVA%20DOS%20SANTOS,%20Bruno&LENG,%20Jacques&OLIVEIRA,%20Hugo&AMEDEE,%20Joelle&rft.genre=article |