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hal.structure.identifierRégulation spatiale des Génomes - Spatial Regulation of Genomes
dc.contributor.authorCHAPARD, Christophe
hal.structure.identifierRégulation spatiale des Génomes - Spatial Regulation of Genomes
hal.structure.identifierCollège Doctoral
dc.contributor.authorMENEU, Léa
hal.structure.identifierRégulation spatiale des Génomes - Spatial Regulation of Genomes
dc.contributor.authorSERIZAY, Jacques
hal.structure.identifierStructure et Instabilité des Génomes [STRING]
hal.structure.identifierCollège Doctoral
dc.contributor.authorWESTBROOK, Alex
hal.structure.identifierStructure et Instabilité des Génomes [STRING]
hal.structure.identifierCollège Doctoral
hal.structure.identifierLaboratoire de Physique Théorique des Liquides [LPTL]
dc.contributor.authorROUTHIER, Etienne
hal.structure.identifierDynamique du noyau [Institut Curie]
dc.contributor.authorRUAULT, Myriam
hal.structure.identifierRégulation spatiale des Génomes - Spatial Regulation of Genomes
hal.structure.identifierCollège Doctoral
dc.contributor.authorBIGNAUD, Amaury
hal.structure.identifierRégulation spatiale des Génomes - Spatial Regulation of Genomes
dc.contributor.authorTHIERRY, Agnès
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGOURGUES, Géraldine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorLARTIGUE, Carole
hal.structure.identifierRégulation spatiale des Génomes - Spatial Regulation of Genomes
dc.contributor.authorPIAZZA, Aurèle
hal.structure.identifierDynamique du noyau [Institut Curie]
dc.contributor.authorTADDEI, Angela
hal.structure.identifierCentre de Biologie Intégrative [CBI]
dc.contributor.authorBECKOUËT, Frédéric
hal.structure.identifierStructure et Instabilité des Génomes [STRING]
hal.structure.identifierLaboratoire de Physique Théorique des Liquides [LPTL]
dc.contributor.authorMOZZICONACCI, Julien
hal.structure.identifierRégulation spatiale des Génomes - Spatial Regulation of Genomes
dc.contributor.authorKOSZUL, Romain
dc.date.issued2022-12-21
dc.description.abstractEnGenomic sequences co-evolve with DNA-associated proteins to ensure the multiscale folding of long DNA molecules into functional chromosomes. In eukaryotes, different molecular complexes organize the chromosome’s hierarchical structure, ranging from nucleosomes and cohesin- mediated DNA loops to large scale chromatin compartments. To explore the relationships between the DNA sequence composition and the spontaneous loading and activity of these DNA-associated complexes in the absence of co-evolution, we characterized chromatin assembly and activity in yeast strains carrying exogenous bacterial chromosomes that diverged from eukaryotic sequences over 1.5 billion years ago. We show that nucleosome assembly, transcriptional activity, cohesin-mediated looping, and chromatin compartmentalization can occur in a bacterial chromosome with a largely divergent sequence integrated in a eukaryotic host, and that the chromatinization of bacterial chromosomes is highly correlated with their sequence composition. These results are a step forward in understanding how foreign sequences are interpreted by a host nuclear machinery during natural horizontal gene transfers, as well as in synthetic genomics projects.
dc.language.isoen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/
dc.title.enExogenous chromosomes reveal how sequence composition drives chromatin assembly, activity, folding and compartmentalization
dc.typeDocument de travail - Pré-publication
dc.identifier.doi10.1101/2022.12.21.520625
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie moléculaire
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Génomique, Transcriptomique et Protéomique [q-bio.GN]
hal.identifierhal-04278801
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-04278801v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.date=2022-12-21&rft.au=CHAPARD,%20Christophe&MENEU,%20L%C3%A9a&SERIZAY,%20Jacques&WESTBROOK,%20Alex&ROUTHIER,%20Etienne&rft.genre=preprint


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