Exogenous chromosomes reveal how sequence composition drives chromatin assembly, activity, folding and compartmentalization
hal.structure.identifier | Régulation spatiale des Génomes - Spatial Regulation of Genomes | |
dc.contributor.author | CHAPARD, Christophe | |
hal.structure.identifier | Régulation spatiale des Génomes - Spatial Regulation of Genomes | |
hal.structure.identifier | Collège Doctoral | |
dc.contributor.author | MENEU, Léa | |
hal.structure.identifier | Régulation spatiale des Génomes - Spatial Regulation of Genomes | |
dc.contributor.author | SERIZAY, Jacques | |
hal.structure.identifier | Structure et Instabilité des Génomes [STRING] | |
hal.structure.identifier | Collège Doctoral | |
dc.contributor.author | WESTBROOK, Alex | |
hal.structure.identifier | Structure et Instabilité des Génomes [STRING] | |
hal.structure.identifier | Collège Doctoral | |
hal.structure.identifier | Laboratoire de Physique Théorique des Liquides [LPTL] | |
dc.contributor.author | ROUTHIER, Etienne | |
hal.structure.identifier | Dynamique du noyau [Institut Curie] | |
dc.contributor.author | RUAULT, Myriam | |
hal.structure.identifier | Régulation spatiale des Génomes - Spatial Regulation of Genomes | |
hal.structure.identifier | Collège Doctoral | |
dc.contributor.author | BIGNAUD, Amaury | |
hal.structure.identifier | Régulation spatiale des Génomes - Spatial Regulation of Genomes | |
dc.contributor.author | THIERRY, Agnès | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | GOURGUES, Géraldine | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | LARTIGUE, Carole | |
hal.structure.identifier | Régulation spatiale des Génomes - Spatial Regulation of Genomes | |
dc.contributor.author | PIAZZA, Aurèle | |
hal.structure.identifier | Dynamique du noyau [Institut Curie] | |
dc.contributor.author | TADDEI, Angela | |
hal.structure.identifier | Centre de Biologie Intégrative [CBI] | |
dc.contributor.author | BECKOUËT, Frédéric | |
hal.structure.identifier | Structure et Instabilité des Génomes [STRING] | |
hal.structure.identifier | Laboratoire de Physique Théorique des Liquides [LPTL] | |
dc.contributor.author | MOZZICONACCI, Julien | |
hal.structure.identifier | Régulation spatiale des Génomes - Spatial Regulation of Genomes | |
dc.contributor.author | KOSZUL, Romain | |
dc.date.issued | 2022-12-21 | |
dc.description.abstractEn | Genomic sequences co-evolve with DNA-associated proteins to ensure the multiscale folding of long DNA molecules into functional chromosomes. In eukaryotes, different molecular complexes organize the chromosome’s hierarchical structure, ranging from nucleosomes and cohesin- mediated DNA loops to large scale chromatin compartments. To explore the relationships between the DNA sequence composition and the spontaneous loading and activity of these DNA-associated complexes in the absence of co-evolution, we characterized chromatin assembly and activity in yeast strains carrying exogenous bacterial chromosomes that diverged from eukaryotic sequences over 1.5 billion years ago. We show that nucleosome assembly, transcriptional activity, cohesin-mediated looping, and chromatin compartmentalization can occur in a bacterial chromosome with a largely divergent sequence integrated in a eukaryotic host, and that the chromatinization of bacterial chromosomes is highly correlated with their sequence composition. These results are a step forward in understanding how foreign sequences are interpreted by a host nuclear machinery during natural horizontal gene transfers, as well as in synthetic genomics projects. | |
dc.language.iso | en | |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/ | |
dc.title.en | Exogenous chromosomes reveal how sequence composition drives chromatin assembly, activity, folding and compartmentalization | |
dc.type | Document de travail - Pré-publication | |
dc.identifier.doi | 10.1101/2022.12.21.520625 | |
dc.subject.hal | Sciences du Vivant [q-bio] | |
dc.subject.hal | Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie moléculaire | |
dc.subject.hal | Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Génomique, Transcriptomique et Protéomique [q-bio.GN] | |
hal.identifier | hal-04278801 | |
hal.version | 1 | |
hal.origin.link | https://hal.archives-ouvertes.fr//hal-04278801v1 | |
bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.date=2022-12-21&rft.au=CHAPARD,%20Christophe&MENEU,%20L%C3%A9a&SERIZAY,%20Jacques&WESTBROOK,%20Alex&ROUTHIER,%20Etienne&rft.genre=preprint |
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