Stochastic pausing at latent HIV-1 promoters generates transcriptional bursting.
| dc.rights.license | open | en_US |
| dc.contributor.author | TANTALE, Katjana | |
| dc.contributor.author | GARCIA-OLIVER, Encar | |
| dc.contributor.author | ROBERT, Marie-Cécile | |
| dc.contributor.author | L'HOSTIS, Adèle | |
| dc.contributor.author | YANG, Yueyuxiao | |
| dc.contributor.author | TSANOV, Nikolay | |
| dc.contributor.author | TOPNO, Rachel | |
| dc.contributor.author | GOSTAN, Thierry | |
| dc.contributor.author | KOZULIC-PIRHER, Alja | |
| dc.contributor.author | BASU-SHRIVASTAVA, Meenakshi | |
| dc.contributor.author | MUKHERJEE, Kamalika | |
| dc.contributor.author | SLANINOVA, Vera | |
| dc.contributor.author | ANDRAU, Jean-Christophe | |
| dc.contributor.author | MUELLER, Florian | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | BASYUK, Eugenia | |
| dc.contributor.author | RADULESCU, Ovidiu | |
| dc.contributor.author | BERTRAND, Edouard | |
| dc.date.accessioned | 2024-10-31T08:20:46Z | |
| dc.date.available | 2024-10-31T08:20:46Z | |
| dc.date.issued | 2021-07-23 | |
| dc.identifier.issn | 2041-1723 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/203065 | |
| dc.description.abstractEn | Promoter-proximal pausing of RNA polymerase II is a key process regulating gene expression. In latent HIV-1 cells, it prevents viral transcription and is essential for latency maintenance, while in acutely infected cells the viral factor Tat releases paused polymerase to induce viral expression. Pausing is fundamental for HIV-1, but how it contributes to bursting and stochastic viral reactivation is unclear. Here, we performed single molecule imaging of HIV-1 transcription. We developed a quantitative analysis method that manages multiple time scales from seconds to days and that rapidly fits many models of promoter dynamics. We found that RNA polymerases enter a long-lived pause at latent HIV-1 promoters (>20 minutes), thereby effectively limiting viral transcription. Surprisingly and in contrast to current models, pausing appears stochastic and not obligatory, with only a small fraction of the polymerases undergoing long-lived pausing in absence of Tat. One consequence of stochastic pausing is that HIV-1 transcription occurs in bursts in latent cells, thereby facilitating latency exit and providing a rationale for the stochasticity of viral rebounds. | |
| dc.language.iso | EN | en_US |
| dc.rights | Attribution 3.0 United States | * |
| dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | * |
| dc.subject.en | Algorithms | |
| dc.subject.en | DNA-Directed RNA Polymerases | |
| dc.subject.en | Gene Expression Regulation | |
| dc.subject.en | Viral | |
| dc.subject.en | HIV Infections | |
| dc.subject.en | HIV-1 | |
| dc.subject.en | HeLa Cells | |
| dc.subject.en | Humans | |
| dc.subject.en | Models | |
| dc.subject.en | Genetic | |
| dc.subject.en | Promoter Regions | |
| dc.subject.en | Genetic | |
| dc.subject.en | Stochastic Processes | |
| dc.subject.en | Time Factors | |
| dc.subject.en | Virus Activation | |
| dc.subject.en | Virus Latency | |
| dc.subject.en | tat Gene Products | |
| dc.subject.en | Human Immunodeficiency Virus | |
| dc.title.en | Stochastic pausing at latent HIV-1 promoters generates transcriptional bursting. | |
| dc.title.alternative | Nat Commun | en_US |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1038/s41467-021-24462-5 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Microbiologie et Parasitologie | en_US |
| dc.identifier.pubmed | 34301927 | en_US |
| bordeaux.journal | Nature Communications | en_US |
| bordeaux.page | 4503 | en_US |
| bordeaux.volume | 12 | en_US |
| bordeaux.hal.laboratories | MFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234 | en_US |
| bordeaux.issue | 1 | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.import.source | pubmed | |
| hal.popular | non | en_US |
| hal.audience | Internationale | en_US |
| hal.export | false | |
| workflow.import.source | pubmed | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Nature%20Communications&rft.date=2021-07-23&rft.volume=12&rft.issue=1&rft.spage=4503&rft.epage=4503&rft.eissn=2041-1723&rft.issn=2041-1723&rft.au=TANTALE,%20Katjana&GARCIA-OLIVER,%20Encar&ROBERT,%20Marie-C%C3%A9cile&L'HOSTIS,%20Ad%C3%A8le&YANG,%20Yueyuxiao&rft.genre=article |
