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Experimental evidence for IS1294b-mediated transposition of the blaCMY-2 cephalosporinase gene in Enterobacteriaceae.
| dc.rights.license | open | en_US |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | BIENTZ, Léa | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | CROS, Jessica | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | GORET, Julien | |
| dc.contributor.author | BÉBÉAR, Cécile | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | QUENTIN, Claudine | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | ARPIN, Corinne
IDREF: 093324626 | |
| dc.date.accessioned | 2024-10-08T08:29:14Z | |
| dc.date.available | 2024-10-08T08:29:14Z | |
| dc.date.issued | 2015-03-01 | |
| dc.identifier.issn | 1460-2091 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/202324 | |
| dc.description.abstractEn | The objective of this study was to investigate whether the insertion sequence IS1294b (IS91 family) is able to mobilize the blaCMY-2 gene and its adjacent regions from one replicon to another. Klebsiella pneumoniae Kp2735 was typed by MLST and its plasmid content was examined by S1-PFGE and PCR-based replicon typing. The genetic blaCMY-2 environment was analysed after cloning experiments and sequencing. Transposition assays were performed with an inactivation strategy based on the sacB gene, which confers sucrose-dependent lethality. Kp2735 (ST215) exhibited high-level resistance to ceftazidime owing to the presence of the cephalosporinase CMY-2. The blaCMY-2 gene was located on an IncI1 ST156 plasmid, p2735, of ∼95 kb. Analysis of the genetic environment revealed, upstream of blaCMY-2, the presence of ISEcp1 interrupted by IS1294b and, downstream of blaCMY-2, a region of 1395 bp belonging to the backbone of IncA/C replicons, suggesting a possible DNA transfer between the two plasmids. We showed that IS1294b is able to mobilize blaCMY-2 and its adjacent regions efficiently on the recipient plasmid with a mean frequency of 5.9%. This transfer was due to a one-ended transposition mechanism, implying the non-recognition of its terIS end. Our experimental data demonstrate for the first time, to our knowledge, the mobilization of a β-lactamase gene mediated by a member of the IS91 family and highlight the important role of this mobile genetic element in the spread of antibiotic resistance genes. | |
| dc.language.iso | EN | en_US |
| dc.subject.en | Anti-Bacterial Agents | |
| dc.subject.en | Ceftazidime | |
| dc.subject.en | Cephalosporinase | |
| dc.subject.en | DNA Transposable Elements | |
| dc.subject.en | DNA | |
| dc.subject.en | Bacterial | |
| dc.subject.en | Humans | |
| dc.subject.en | Klebsiella Infections | |
| dc.subject.en | Klebsiella pneumoniae | |
| dc.subject.en | Molecular Sequence Data | |
| dc.subject.en | Plasmids | |
| dc.subject.en | Recombination | |
| dc.subject.en | Genetic | |
| dc.subject.en | Replicon | |
| dc.subject.en | Sequence Analysis | |
| dc.subject.en | DNA | |
| dc.subject.en | beta-Lactam Resistance | |
| dc.subject.en | beta-Lactamases | |
| dc.title.en | Experimental evidence for IS1294b-mediated transposition of the blaCMY-2 cephalosporinase gene in Enterobacteriaceae. | |
| dc.title.alternative | J Antimicrob Chemother | en_US |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1093/jac/dku472 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Microbiologie et Parasitologie | en_US |
| dc.identifier.pubmed | 25428924 | en_US |
| bordeaux.journal | Journal of Antimicrobial Chemotherapy | en_US |
| bordeaux.page | 697-700 | en_US |
| bordeaux.volume | 70 | en_US |
| bordeaux.hal.laboratories | MFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234 | en_US |
| bordeaux.issue | 3 | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.import.source | pubmed | |
| hal.identifier | hal-04725328 | |
| hal.version | 1 | |
| hal.date.transferred | 2024-10-08T08:29:17Z | |
| hal.popular | non | en_US |
| hal.audience | Internationale | en_US |
| hal.export | true | |
| workflow.import.source | pubmed | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20Antimicrobial%20Chemotherapy&rft.date=2015-03-01&rft.volume=70&rft.issue=3&rft.spage=697-700&rft.epage=697-700&rft.eissn=1460-2091&rft.issn=1460-2091&rft.au=BIENTZ,%20L%C3%A9a&CROS,%20Jessica&GORET,%20Julien&B%C3%89B%C3%89AR,%20C%C3%A9cile&QUENTIN,%20Claudine&rft.genre=article |
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