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Structure of a DNA G-quadruplex that Modulates SP1 Binding Sites Architecture in HIV-1 Promoter.

dc.rights.licenseopenen_US
hal.structure.identifierRégulations Naturelles et Artificielles [ARNA]
dc.contributor.authorDE RACHE, Aurore
hal.structure.identifierRégulations Naturelles et Artificielles [ARNA]
dc.contributor.authorMARQUEVIELLE, Julien
dc.contributor.authorBOUAZIZ, Serge
hal.structure.identifierRégulations Naturelles et Artificielles [ARNA]
dc.contributor.authorVIALET, Brune
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorANDREOLA, Marie-Line
dc.contributor.authorMERGNY, Jean-Louis
hal.structure.identifierRégulations Naturelles et Artificielles [ARNA]
dc.contributor.authorAMRANE, Samir
dc.date.accessioned2024-10-07T10:43:49Z
dc.date.available2024-10-07T10:43:49Z
dc.date.issued2024-01-15
dc.identifier.issn1089-8638en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/202286
dc.description.abstractEnNucleic acid sequences containing guanine tracts are able to form non-canonical DNA or RNA structures known as G-quadruplexes (or G4s). These structures, based on the stacking of G-tetrads, are involved in various biological processes such as gene expression regulation. Here, we investigated a G4 forming sequence, HIVpro2, derived from the HIV-1 promoter. This motif is located 60 nucleotides upstream of the proviral Transcription Starting Site (TSS) and overlaps with two SP1 transcription factor binding sites. Using NMR spectroscopy, we determined that HIVpro2 forms a hybrid type G4 structure with a core that is interrupted by a single nucleotide bulge. An additional reverse-Hoogsteen AT base pair is stacked on top of the tetrad. SP1 transcription factor is known to regulate transcription activity of many genes through the recognition of Guanine-rich duplex motifs. Here, the formation of HIVpro2 G4 may modulate SP1 binding sites architecture by competing with the formation of the canonical duplex structure. Such DNA structural switch potentially participates to the regulation of viral transcription and may also interfere with HIV-1 reactivation or viral latency.
dc.language.isoENen_US
dc.subject.enBinding Sites
dc.subject.enDNA
dc.subject.enG-Quadruplexes
dc.subject.enGuanine
dc.subject.enHIV-1
dc.subject.enSp1 Transcription Factor
dc.subject.enHumans
dc.subject.enGene Expression Regulation
dc.subject.enViral
dc.title.enStructure of a DNA G-quadruplex that Modulates SP1 Binding Sites Architecture in HIV-1 Promoter.
dc.title.alternativeJ Mol Biolen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1016/j.jmb.2023.168359en_US
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologieen_US
dc.identifier.pubmed37952768en_US
bordeaux.journalJournal of Molecular Biologyen_US
bordeaux.page168359en_US
bordeaux.volume436en_US
bordeaux.hal.laboratoriesMFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234en_US
bordeaux.issue2en_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcepubmed
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
workflow.import.sourcepubmed
dc.rights.ccPas de Licence CCen_US
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