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Functional single-cell analyses of mesenchymal stromal cell proliferation and differentiation using ALDH-activity and mitochondrial ROS content.

dc.rights.licenseopenen_US
hal.structure.identifierBiothérapies des maladies génétiques et cancers
hal.structure.identifierLaboratoire Maladies Rares: Génétique et Métabolisme (Bordeaux) [U1211 INSERM/MRGM]
hal.structure.identifierEtablissement Français du Sang Nouvelle Aquitaine [Bordeaux] [EFS Bordeaux Nouvelle Aquitaine]
dc.contributor.authorREFEYTON, Alice
hal.structure.identifierLaboratoire Maladies Rares: Génétique et Métabolisme (Bordeaux) [U1211 INSERM/MRGM]
hal.structure.identifierEtablissement Français du Sang Nouvelle Aquitaine [Bordeaux] [EFS Bordeaux Nouvelle Aquitaine]
dc.contributor.authorLABAT, Veronique
hal.structure.identifierEtablissement Français du Sang Nouvelle Aquitaine [Bordeaux] [EFS Bordeaux Nouvelle Aquitaine]
dc.contributor.authorMOMBLED, Margaux
hal.structure.identifierLaboratoire Maladies Rares: Génétique et Métabolisme (Bordeaux) [U1211 INSERM/MRGM]
hal.structure.identifierEtablissement Français du Sang Nouvelle Aquitaine [Bordeaux] [EFS Bordeaux Nouvelle Aquitaine]
dc.contributor.authorVLASKI-LAFARGE, Marija
hal.structure.identifierLaboratoire Maladies Rares: Génétique et Métabolisme (Bordeaux) [U1211 INSERM/MRGM]
hal.structure.identifierEtablissement Français du Sang Nouvelle Aquitaine [Bordeaux] [EFS Bordeaux Nouvelle Aquitaine]
dc.contributor.authorIVANOVIC, Zoran
dc.date.accessioned2024-10-04T13:26:18Z
dc.date.available2024-10-04T13:26:18Z
dc.date.issued2024-08-01
dc.identifier.issn1477-2566en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/202263
dc.description.abstractEnPrevious research has unveiled a stem cell-like transcriptome enrichment in the aldehyde dehydrogenase-expressing (ALDH) mesenchymal stromal cell (MStroC) fraction. However, considering the heterogeneity of MStroCs, with only a fraction of them presenting bona fide stem cells (MSCs), the actual potency of ALDH as an MSC-specific selection marker remains an issue. To address this, the proliferative and differentiation potential of individual ALDH and ALDH MStroCs incubated at low oxygen concentrations, estimated to mimic stem cell niches (0.1% O), were assayed using single-cell clonal analysis, compared to standard conditions (20% O). We confirm that a high proliferative capacity and multi-potent MSCs are enriched in the ALDH MStroC population, especially when cells are cultured at 0.1% O. Measurements of reduced/oxidized glutathione and mitochondrial superoxide anions with MitoSoX (MSX) indicate that this advantage induced by low oxygen is related to a decrease in the oxidative and reactive oxygen species (ROS) levels in the stem cell metabolic setup. However, ALDH expression is neither specific nor exclusive to MSCs, as high proliferative capacity and multi-potent cells were also found in the ALDH fraction. Furthermore, single-cell assays performed after combined cell sorting based on ALDH and MSX showed that the MSX MStroC population is enriched in stem/progenitor cells in all conditions, irrespective of ALDH expression or culture oxygen concentration. Importantly, the ALDHMSX MStroC fraction exposed to 0.1% O was almost exclusively composed of genuine MSCs. In contrast, neither progenitors nor stem cells (with a complete absence of colony-forming ability) were detected in the MSX fraction, which exclusively resides in the ALDH MStroC population. Our study reveals that ALDH expression is not exclusively associated with MSCs. However, cell sorting using combined ALDH expression and ROS content can be utilized to exclude MStroCs lacking stem/progenitor cell properties.
dc.language.isoENen_US
dc.subjectAldehyde dehydrogenase
dc.subjectMesenchymal stem cells
dc.subjectMulti-potency
dc.subjectOxygen concentration
dc.subjectROS content
dc.subjectSingle cell analysis
dc.title.enFunctional single-cell analyses of mesenchymal stromal cell proliferation and differentiation using ALDH-activity and mitochondrial ROS content.
dc.title.alternativeCytotherapyen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1016/j.jcyt.2024.04.003en_US
dc.subject.halSciences du Vivant [q-bio]/Génétiqueen_US
dc.identifier.pubmed38661612en_US
bordeaux.journalCytotherapyen_US
bordeaux.page813-824en_US
bordeaux.volume26en_US
bordeaux.hal.laboratoriesMaladies Rares : Génétique et Métabolisme (MRGM) - UMR 1211en_US
bordeaux.issue8en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionINSERMen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.identifier.funderIDInstitut National de la Santé et de la Recherche Médicaleen_US
bordeaux.import.sourcepubmed
hal.identifierhal-04721816
hal.version1
hal.date.transferred2024-10-04T13:26:21Z
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exporttrue
workflow.import.sourcepubmed
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Cytotherapy&rft.date=2024-08-01&rft.volume=26&rft.issue=8&rft.spage=813-824&rft.epage=813-824&rft.eissn=1477-2566&rft.issn=1477-2566&rft.au=REFEYTON,%20Alice&LABAT,%20Veronique&MOMBLED,%20Margaux&VLASKI-LAFARGE,%20Marija&IVANOVIC,%20Zoran&rft.genre=article


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