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Streptococcus pyogenes Cas9 ribonucleoprotein delivery for efficient, rapid and marker-free gene editing in Trypanosoma and Leishmania.

dc.rights.licenseopenen_US
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorASENCIO, Corinne
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorHERVÉ, Perrine
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorMORAND, Pauline
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorOLIVERES, Quentin
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorMOREL, Chloé Alexandra
hal.structure.identifierTBM-Core [Bordeaux] [UMS3427 - INSERM US005]
dc.contributor.authorPROUZET-MAULEON, Valérie
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorBIRAN, Marc
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorMONIC, Sarah
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorBONHIVERS, Mélanie
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorROBINSON, Derrick Roy
IDREF: 11410350X
dc.contributor.authorOUELLETTE, Marc
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorRIVIÈRE, Loïc
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorBRINGAUD, Frédéric
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorTETAUD, Emmanuel
IDREF: 25436697X
dc.date.accessioned2024-10-01T09:16:05Z
dc.date.available2024-10-01T09:16:05Z
dc.date.issued2024-06-01
dc.identifier.issn1365-2958en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/202081
dc.description.abstractEnKinetoplastids are unicellular eukaryotic flagellated parasites found in a wide range of hosts within the animal and plant kingdoms. They are known to be responsible in humans for African sleeping sickness (Trypanosoma brucei), Chagas disease (Trypanosoma cruzi), and various forms of leishmaniasis (Leishmania spp.), as well as several animal diseases with important economic impact (African trypanosomes, including Trypanosoma congolense). Understanding the biology of these parasites necessarily implies the ability to manipulate their genomes. In this study, we demonstrate that transfection of a ribonucleoprotein complex, composed of recombinant Streptococcus pyogenes Cas9 (SpCas9) and an in vitro-synthesized guide RNA, results in rapid and efficient genetic modifications of trypanosomatids, in marker-free conditions. This approach was successfully developed to inactivate, delete, and mutate candidate genes in various stages of the life cycle of T. brucei and T. congolense, and Leishmania promastigotes. The functionality of SpCas9 in these parasites now provides, to the research community working on these parasites, a rapid and efficient method of genome editing, without requiring plasmid construction and selection by antibiotics but requires only cloning and PCR screening of the clones. Importantly, this approach is adaptable to any wild-type parasite.
dc.language.isoENen_US
dc.subject.enGene Editing
dc.subject.enCRISPR-Cas Systems
dc.subject.enRibonucleoproteins
dc.subject.enStreptococcus pyogenes
dc.subject.enLeishmania
dc.subject.enCRISPR-Associated Protein 9
dc.subject.enRNA
dc.subject.enGuide
dc.subject.enCRISPR-Cas Systems
dc.subject.enTrypanosoma brucei brucei
dc.subject.enTrypanosoma
dc.subject.enTransfection
dc.title.enStreptococcus pyogenes Cas9 ribonucleoprotein delivery for efficient, rapid and marker-free gene editing in Trypanosoma and Leishmania.
dc.title.alternativeMol Microbiolen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1111/mmi.15256en_US
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologieen_US
dc.identifier.pubmed38558208en_US
bordeaux.journalMolecular Microbiologyen_US
bordeaux.page1079-1094en_US
bordeaux.volume121en_US
bordeaux.hal.laboratoriesMFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234en_US
bordeaux.issue6en_US
bordeaux.institutionCNRSen_US
bordeaux.institutionUniversité de Bordeaux
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcepubmed
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
workflow.import.sourcepubmed
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Molecular%20Microbiology&rft.date=2024-06-01&rft.volume=121&rft.issue=6&rft.spage=1079-1094&rft.epage=1079-1094&rft.eissn=1365-2958&rft.issn=1365-2958&rft.au=ASENCIO,%20Corinne&HERV%C3%89,%20Perrine&MORAND,%20Pauline&OLIVERES,%20Quentin&MOREL,%20Chlo%C3%A9%20Alexandra&rft.genre=article


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