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First step towards whole genome cloning of Bacillus subtilis in yeast by CReasPy-Fusion

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorJULIEN, Barret
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGUESDON, Gabrielle
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGOURGUES, Geraldine
hal.structure.identifierMICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
dc.contributor.authorPLANSON, Anne-Gaëlle
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorSIRAND-PUGNET, Pascal
hal.structure.identifierMICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
dc.contributor.authorSAUVEPLANE, V.
hal.structure.identifierMICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
dc.contributor.authorDERVYN, Etienne
hal.structure.identifierMICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
dc.contributor.authorJULES, Matthieu
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierMICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
dc.contributor.authorLARTIGUE, Carole
dc.date.conference2023-06-27
dc.description.abstractEnIn 2010, promising synthetic biology technologies have emerged that use yeast as a platform for the assembly and engineering of synthetic bacterial genomes prior their transplantation into a recipient cell. These technologies opened up new avenues towards the construction of cells with fully controlled biological properties.The transfer of these tools to microorganisms of industrial interest, such as the Gram+ bacterium Bacillus subtilis (Bsu), will be a central advance in the field of biotechnology. The INRAE consortium has set out to clone the whole Bsu genome in yeast using CReasPy-Fusion, a newly developed method based on direct fusion between bacterial protoplasts and yeast spheroplasts preloaded with a CRISPR Cas9 tool. Efforts to date have demonstrated: (1) cell-to-cell fusion between Bsu and yeast, a phenomenon never described before; (2) the efficiency of a CRISPR Cas9 system to capture and modify a shuttle plasmid during Bsu/yeast fusion; and (3) the efficiency of the CRISPR Cas9 system to capture a 130-kb fragment of the Bsu genome. Since then, larger Bsu genome fragments have been cloned in yeast; their capture being facilitated by the addition of ARS elements along the Bsu chromosome. We now aim to clone the ~3 Mb genome of a genome-reduced Bsu strain in yeast.
dc.description.sponsorshipConstruction et transplantation de génomes semi-synthétiques de Bacillus subtilis, vers le développement de la prochaine génération de châssis bactériens - ANR-18-CE44-0003
dc.language.isoen
dc.title.enFirst step towards whole genome cloning of Bacillus subtilis in yeast by CReasPy-Fusion
dc.typeAutre communication scientifique (congrès sans actes - poster - séminaire...)
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie
bordeaux.conference.titleCRISPR conference 2023
bordeaux.countryDE
bordeaux.conference.cityWürzburg
bordeaux.peerReviewedoui
hal.identifierhal-04693946
hal.version1
hal.invitednon
hal.proceedingsnon
hal.conference.end2023-07-01
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-04693946v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.au=JULIEN,%20Barret&GUESDON,%20Gabrielle&GOURGUES,%20Geraldine&PLANSON,%20Anne-Ga%C3%ABlle&SIRAND-PUGNET,%20Pascal&rft.genre=conference


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