JULIEN, Barret; GUESDON, Gabrielle; GOURGUES, Geraldine; PLANSON, Anne-Gaëlle; SIRAND-PUGNET, Pascal; SAUVEPLANE, Vincent; DERVYN, Etienne; JULES, Matthieu; LARTIGUE, Carole

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JULIEN, Barret
Biologie du fruit et pathologie [BFP]

GUESDON, Gabrielle
Biologie du fruit et pathologie [BFP]

GOURGUES, Geraldine
Biologie du fruit et pathologie [BFP]

Langue

Autre communication scientifique (congrès sans actes - poster - séminaire...)

Ce document a été publié dans

CRISPR conference 2023, 2023-06-27, Würzburg.

Résumé en anglais

In 2010, promising synthetic biology technologies have emerged that use yeast as a platform for the assembly and engineering of synthetic bacterial genomes prior their transplantation into a recipient cell. These technologies opened up new avenues towards the construction of cells with fully controlled biological properties.The transfer of these tools to microorganisms of industrial interest, such as the Gram+ bacterium Bacillus subtilis (Bsu), will be a central advance in the field of biotechnology. The INRAE consortium has set out to clone the whole Bsu genome in yeast using CReasPy-Fusion, a newly developed method based on direct fusion between bacterial protoplasts and yeast spheroplasts preloaded with a CRISPR Cas9 tool. Efforts to date have demonstrated: (1) cell-to-cell fusion between Bsu and yeast, a phenomenon never described before; (2) the efficiency of a CRISPR Cas9 system to capture and modify a shuttle plasmid during Bsu/yeast fusion; and (3) the efficiency of the CRISPR Cas9 system to capture a 130-kb fragment of the Bsu genome. Since then, larger Bsu genome fragments have been cloned in yeast; their capture being facilitated by the addition of ARS elements along the Bsu chromosome. We now aim to clone the ~3 Mb genome of a genome-reduced Bsu strain in yeast.< Réduire

URI

https://oskar-bordeaux.fr/handle/20.500.12278/201548

Project ANR

Construction et transplantation de génomes semi-synthétiques de Bacillus subtilis, vers le développement de la prochaine génération de châssis bactériens - ANR-18-CE44-0003

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Importé de hal

Unités de recherche

Biologie du Fruit & Pathologie (BFP) - UMR 1332