LARTIGUE, Carole; SIRAND-PUGNET, Pascal

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LARTIGUE, Carole
Biologie du fruit et pathologie [BFP]

SIRAND-PUGNET, Pascal
Biologie du fruit et pathologie [BFP]

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Communication dans un congrès

Ce document a été publié dans

Minimal Cell Workshop (virtual), 2022-09-22, San Diego.

Résumé en anglais

Due to the lack of efficient recombination in most species, production of mutant strains and genome engineering of mycoplasmas and other mollicutes has long been limited. More than a decade ago, the first successful cloning of a mycoplasma genome in yeast and subsequent back transplantation into a recipient cell opened undreamed possibilities of bacterial genome manipulations. From that time, 20 natural genomes from 15 species of mollicutes have been cloned in yeast, using various protocols as the newly released CreasPy-cloning and CreasPy-Fusion methods. Genome transplantation remains the main bottleneck and is currently available for seven species, related to the so-called Mycoplasma mycoides cluster. Our current work aims to identify the main barriers limiting the expansion of genome transplantation to other species. In parallel, several new genetic tools have been recently developed for gene targeting directly in mycoplasma cells. These include an exogenous recombination RecET system, a CRISPR base editor and a novel strategy for genome engineering based on the recombinase-assisted genomic engineering (RAGE) technology.< Réduire

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Unités de recherche

Biologie du Fruit & Pathologie (BFP) - UMR 1332