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Characterization of six ampeloviruses infecting pineapple in Reunion Island using a combination of high-throughput sequencing approaches

hal.structure.identifierPeuplements végétaux et bioagresseurs en milieu tropical [UMR PVBMT]
hal.structure.identifierUnité Ravageurs et agents pathogènes tropicaux [RAPT]
dc.contributor.authorMASSÉ, Delphine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCANDRESSE, Thierry
hal.structure.identifierPlant Health Institute of Montpellier [UMR PHIM]
dc.contributor.authorFILLOUX, Denis
hal.structure.identifierGembloux Agro-Bio Tech [Faculté universitaire des sciences agronomiques de Gembloux] [[FUSAGx]]
dc.contributor.authorMASSART, Sébastien
hal.structure.identifierUnité Ravageurs et agents pathogènes tropicaux [RAPT]
dc.contributor.authorCASSAM, Nathalie
hal.structure.identifierUnité Ravageurs et agents pathogènes tropicaux [RAPT]
dc.contributor.authorHOSTACHY, Bruno
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMARAIS, Armelle
hal.structure.identifierPlant Health Institute of Montpellier [UMR PHIM]
dc.contributor.authorFERNANDEZ, Emmanuel
hal.structure.identifierPlant Health Institute of Montpellier [UMR PHIM]
dc.contributor.authorROUMAGNAC, Philippe
hal.structure.identifierUnité de Pathologie Végétale [PV]
dc.contributor.authorVERDIN, Eric
hal.structure.identifierPeuplements végétaux et bioagresseurs en milieu tropical [UMR PVBMT]
dc.contributor.authorTEYCHENEY, Pierre-Yves
hal.structure.identifierPeuplements végétaux et bioagresseurs en milieu tropical [UMR PVBMT]
dc.contributor.authorLETT, Jean-Michel
hal.structure.identifierPeuplements végétaux et bioagresseurs en milieu tropical [UMR PVBMT]
dc.contributor.authorLEFEUVRE, Pierre
dc.date.issued2024-07-16
dc.identifier.issn1999-4915
dc.description.abstractEnThe cultivation of pineapple (Ananas comosus) is threatened worldwide by mealybug wilt disease of pineapple (MWP), whose etiology is not yet fully elucidated. In this study, we characterized pineapple mealybug wilt-associated ampeloviruses (PMWaVs, family Closteroviridae) from a diseased pineapple plant collected from Reunion Island, using a high-throughput sequencing approach combining Illumina short reads and Nanopore long reads. Reads co-assembly resulted in complete or near-complete genomes for six distinct ampeloviruses, including the first complete genome of pineapple mealybug wilt-associated virus 5 (PMWaV5) and that of a new species tentatively named pineapple mealybug wilt-associated virus 7 (PMWaV7). Short reads data provided high genome coverage and sequencing depths for all six viral genomes, contrary to long reads data. The 5′ and 3′ ends of the genome for most of the six ampeloviruses could be recovered from long reads, providing an alternative to RACE-PCRs. Phylogenetic analyses did not unveil any geographic structuring of the diversity of PMWaV1, PMWaV2 and PMWaV3 isolates, supporting the current hypothesis that PMWaVs were mainly spread by human activity and vegetative propagation.
dc.language.isoen
dc.publisherMDPI
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subject.enAnanas comosus
dc.subject.enpineapple mealybug wilt-associated viruses
dc.subject.enampelovirus
dc.subject.enhigh-throughput sequencing
dc.subject.enshort and long reads
dc.subject.enmolecular diversity
dc.title.enCharacterization of six ampeloviruses infecting pineapple in Reunion Island using a combination of high-throughput sequencing approaches
dc.typeArticle de revue
dc.identifier.doi10.3390/v16071146
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie
bordeaux.journalViruses
bordeaux.page1146
bordeaux.volume16
bordeaux.issue7
bordeaux.peerReviewedoui
hal.identifierhal-04655385
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-04655385v1
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