Characterization of six ampeloviruses infecting pineapple in Reunion Island using a combination of high-throughput sequencing approaches
MASSÉ, Delphine
Peuplements végétaux et bioagresseurs en milieu tropical [UMR PVBMT]
Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail [ANSES]
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Peuplements végétaux et bioagresseurs en milieu tropical [UMR PVBMT]
Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail [ANSES]
MASSÉ, Delphine
Peuplements végétaux et bioagresseurs en milieu tropical [UMR PVBMT]
Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail [ANSES]
Peuplements végétaux et bioagresseurs en milieu tropical [UMR PVBMT]
Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail [ANSES]
CASSAM, Nathalie
Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail [ANSES]
Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail [ANSES]
HOSTACHY, Bruno
Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail [ANSES]
< Reduce
Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail [ANSES]
Language
en
Article de revue
This item was published in
Viruses. 2024-07-16, vol. 16, n° 7, p. 1146
MDPI
English Abstract
The cultivation of pineapple (Ananas comosus) is threatened worldwide by mealybug wilt disease of pineapple (MWP), whose etiology is not yet fully elucidated. In this study, we characterized pineapple mealybug wilt-associated ...Read more >
The cultivation of pineapple (Ananas comosus) is threatened worldwide by mealybug wilt disease of pineapple (MWP), whose etiology is not yet fully elucidated. In this study, we characterized pineapple mealybug wilt-associated ampeloviruses (PMWaVs, family Closteroviridae) from a diseased pineapple plant collected from Reunion Island, using a high-throughput sequencing approach combining Illumina short reads and Nanopore long reads. Reads co-assembly resulted in complete or near-complete genomes for six distinct ampeloviruses, including the first complete genome of pineapple mealybug wilt-associated virus 5 (PMWaV5) and that of a new species tentatively named pineapple mealybug wilt-associated virus 7 (PMWaV7). Short reads data provided high genome coverage and sequencing depths for all six viral genomes, contrary to long reads data. The 5′ and 3′ ends of the genome for most of the six ampeloviruses could be recovered from long reads, providing an alternative to RACE-PCRs. Phylogenetic analyses did not unveil any geographic structuring of the diversity of PMWaV1, PMWaV2 and PMWaV3 isolates, supporting the current hypothesis that PMWaVs were mainly spread by human activity and vegetative propagation.Read less <
English Keywords
Ananas comosus
pineapple mealybug wilt-associated viruses
ampelovirus
high-throughput sequencing
short and long reads
molecular diversity
Origin
Hal importedCollections