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dc.rights.licenseopenen_US
dc.contributor.authorDUBAYLE, David
dc.contributor.authorVANDEN-BOSSCHE, Arnaud
hal.structure.identifierInstitut de Neurosciences cognitives et intégratives d'Aquitaine [INCIA]
hal.structure.identifierInstitut des Maladies Neurodégénératives [Bordeaux] [IMN]
dc.contributor.authorPEIXOTO, Tom
hal.structure.identifierInstitut de Neurosciences cognitives et intégratives d'Aquitaine [INCIA]
hal.structure.identifierInstitut des Maladies Neurodégénératives [Bordeaux] [IMN]
dc.contributor.authorMOREL, Jean Luc
IDREF: 113377533
dc.date.accessioned2024-05-27T09:47:50Z
dc.date.available2024-05-27T09:47:50Z
dc.date.issued2023-02-24
dc.identifier.issn2073-4409en_US
dc.identifier.otherhttps: //www.mdpi.com/article/10.3390/cells12050734/s1en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/200059
dc.description.abstractEnThe earliest effect of spaceflight is an alteration in vestibular function due to microgravity. Hypergravity exposure induced by centrifugation is also able to provoke motion sickness. The blood-brain barrier (BBB) is the crucial interface between the vascular system and the brain to ensure efficient neuronal activity. We developed experimental protocols of hypergravity on C57Bl/6JRJ mice to induce motion sickness and reveal its effects on the BBB. Mice were centrifuged at 2× for 24 h. Fluorescent dextrans with different sizes (40, 70 and 150 kDa) and fluorescent antisense oligonucleotides (AS) were injected into mice retro-orbitally. The presence of fluorescent molecules was revealed by epifluorescence and confocal microscopies in brain slices. Gene expression was evaluated by RT-qPCR from brain extracts. Only the 70 kDa dextran and AS were detected in the parenchyma of several brain regions, suggesting an alteration in the BBB. Moreover, Ctnnd1, Gja4 and Actn1 were upregulated, whereas Jup, Tjp2, Gja1, Actn2, Actn4, Cdh2 and Ocln genes were downregulated, specifically suggesting a dysregulation in the tight junctions of endothelial cells forming the BBB. Our results confirm the alteration in the BBB after a short period of hypergravity exposure.
dc.description.sponsorshipDéveloppement d'une infrastructure française distribuée coordonnée - ANR-10-INBS-0004
dc.language.isoENen_US
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.subject.enantisense oligonucleotide
dc.subject.enblood–brain barrier
dc.subject.encentrifugation
dc.subject.endextran
dc.subject.enhypergravity
dc.subject.enpermeability
dc.title.enHypergravity Increases Blood-Brain Barrier Permeability to Fluorescent Dextran and Antisense Oligonucleotide in Mice
dc.title.alternativeCellsen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.3390/cells12050734en_US
dc.subject.halSciences du Vivant [q-bio]/Neurosciences [q-bio.NC]en_US
dc.identifier.pubmed36899870en_US
bordeaux.journalCellsen_US
bordeaux.page734en_US
bordeaux.volume12en_US
bordeaux.hal.laboratoriesInstitut de neurosciences cognitives et intégratives d'Aquitaine (INCIA) - UMR 5287en_US
bordeaux.issue5en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.identifier.funderIDCentre National d’Etudes Spatialesen_US
bordeaux.identifier.funderIDAgence Nationale de la Rechercheen_US
bordeaux.identifier.funderIDCentre National de la Recherche Scientifiqueen_US
bordeaux.identifier.funderIDUniversité Sorbonne Paris Citéen_US
bordeaux.identifier.funderIDUniversité de Bordeauxen_US
bordeaux.import.sourcepubmed
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
workflow.import.sourcepubmed
dc.rights.ccCC BYen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Cells&rft.date=2023-02-24&rft.volume=12&rft.issue=5&rft.spage=734&rft.epage=734&rft.eissn=2073-4409&rft.issn=2073-4409&rft.au=DUBAYLE,%20David&VANDEN-BOSSCHE,%20Arnaud&PEIXOTO,%20Tom&MOREL,%20Jean%20Luc&rft.genre=article


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