Bcl-xL Is Spontaneously Inserted into Preassembled Nanodiscs and Stimulates Bax Insertion in a Cell-Free Protein Synthesis System
| dc.rights.license | open | en_US |
| hal.structure.identifier | Institut de biochimie et génétique cellulaires [IBGC] | |
| dc.contributor.author | ROUCHIDANE EYITAYO, Akande | |
| hal.structure.identifier | Institut de biochimie et génétique cellulaires [IBGC] | |
| dc.contributor.author | BOUDIER-LEMOSQUET, Axel | |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | CHAIGNEPAIN, Stephane | |
| hal.structure.identifier | Institut de biochimie et génétique cellulaires [IBGC] | |
| dc.contributor.author | PRIAULT, Muriel | |
| hal.structure.identifier | Institut de biochimie et génétique cellulaires [IBGC] | |
| dc.contributor.author | MANON, Stephen | |
| dc.date.accessioned | 2024-05-02T08:09:40Z | |
| dc.date.available | 2024-05-02T08:09:40Z | |
| dc.date.issued | 2023 | |
| dc.identifier.issn | 2218-273X | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/199564 | |
| dc.description.abstractEn | The antiapoptotic protein Bcl-xL is a major regulator of cell death and survival, but many aspects of its functions remain elusive. It is mostly localized in the mitochondrial outer membrane (MOM) owing to its C-terminal hydrophobic α-helix. In order to gain further information about its membrane organization, we set up a model system combining cell-free protein synthesis and nanodisc insertion. We found that, contrary to its proapoptotic partner Bax, neosynthesized Bcl-xL was spontaneously inserted into nanodiscs. The deletion of the C-terminal α-helix of Bcl-xL prevented nanodisc insertion. We also found that nanodisc insertion protected Bcl-xL against the proteolysis of the 13 C-terminal residues that occurs during expression of Bcl-xL as a soluble protein in E. coli. Interestingly, we observed that Bcl-xL increased the insertion of Bax into nanodiscs, in a similar way to that which occurs in mitochondria. Cell-free synthesis in the presence of nanodiscs is, thus, a suitable model system to study the molecular aspects of the interaction between Bcl-xL and Bax during their membrane insertion. | |
| dc.language.iso | EN | en_US |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/ | |
| dc.subject.en | apoptosis | |
| dc.subject.en | Bcl-2 family | |
| dc.subject.en | nanodiscs | |
| dc.title.en | Bcl-xL Is Spontaneously Inserted into Preassembled Nanodiscs and Stimulates Bax Insertion in a Cell-Free Protein Synthesis System | |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.3390/biom13060876 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire | en_US |
| bordeaux.journal | Biomolecules | en_US |
| bordeaux.page | 876 | en_US |
| bordeaux.volume | 13 | en_US |
| bordeaux.hal.laboratories | CBMN : Chimie & de Biologie des Membranes & des Nano-objets - UMR 5248 | en_US |
| bordeaux.issue | 6 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | Bordeaux INP | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.import.source | hal | |
| hal.identifier | hal-04255612 | |
| hal.version | 1 | |
| hal.popular | non | en_US |
| hal.audience | Internationale | en_US |
| hal.export | false | |
| workflow.import.source | hal | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biomolecules&rft.date=2023&rft.volume=13&rft.issue=6&rft.spage=876&rft.epage=876&rft.eissn=2218-273X&rft.issn=2218-273X&rft.au=ROUCHIDANE%20EYITAYO,%20Akande&BOUDIER-LEMOSQUET,%20Axel&CHAIGNEPAIN,%20Stephane&PRIAULT,%20Muriel&MANON,%20Stephen&rft.genre=article |
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