Assembly mechanism and cryoEM structure of RecA recombination nucleofilaments from Streptococcus pneumoniae
| dc.rights.license | open | en_US |
| hal.structure.identifier | Unité de biologie Moléculaire, Cellulaire et du Développement [MCD] | |
| dc.contributor.author | HERTZOG, Maud | |
| hal.structure.identifier | Acides Nucléiques : Régulations Naturelle et Artificielle [ARNA] | |
| dc.contributor.author | PERRY, Thomas | |
| hal.structure.identifier | Intégrité du génome et cancers [IGC] | |
| dc.contributor.author | DUPAIGNE, Pauline | |
| hal.structure.identifier | Synthèse et Physico-Chimie de Molécules d'Intérêt Biologique [SPCMIB] | |
| dc.contributor.author | SERRES, Sandra | |
| hal.structure.identifier | Laboratoire de microbiologie et génétique moléculaires - UMR5100 [LMGM] | |
| hal.structure.identifier | Université Toulouse III - Paul Sabatier [UT3] | |
| dc.contributor.author | MORALES, Violette | |
| hal.structure.identifier | Laboratoire de microbiologie et génétique moléculaires - UMR5100 [LMGM] | |
| dc.contributor.author | SOULET, Anne-Lise | |
| hal.structure.identifier | University of Waterloo [Waterloo] | |
| dc.contributor.author | BELL, Jason | |
| hal.structure.identifier | Centre de Biologie Structurale [Montpellier] [CBS] | |
| dc.contributor.author | MARGEAT, Emmanuel | |
| hal.structure.identifier | University of California [UC] | |
| dc.contributor.author | KOWALCZYKOWSKI, Stephen | |
| hal.structure.identifier | Intégrité du génome et cancers [IGC] | |
| dc.contributor.author | LE CAM, Eric | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | FRONZES, Remi | |
| hal.structure.identifier | Laboratoire de microbiologie et génétique moléculaires - UMR5100 [LMGM] | |
| dc.contributor.author | POLARD, Patrice | |
| dc.date.accessioned | 2024-04-24T08:59:56Z | |
| dc.date.available | 2024-04-24T08:59:56Z | |
| dc.date.issued | 2023-02-21 | |
| dc.identifier.issn | 0305-1048 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/199292 | |
| dc.description.abstractEn | RecA-mediated homologous recombination (HR) is a key mechanism for genome maintenance and plasticity in bacteria. It proceeds through RecA assembly into a dynamic filament on ssDNA, the presynaptic filament, which mediates DNA homology search and ordered DNA strand exchange. Here, we combined structural, single molecule and biochemical approaches to characterize the ATP-dependent assembly mechanism of the presynaptic filament of RecA from Streptococcus pneumoniae (SpRecA), in comparison to the Escherichia coli RecA (EcRecA) paradigm. EcRecA polymerization on ssDNA is assisted by the Single-Stranded DNA Binding (SSB) protein, which unwinds ssDNA secondary structures that block EcRecA nucleofilament growth. We report by direct microscopic analysis of SpRecA filamentation on ssDNA that neither of the two paralogous pneumococcal SSBs could assist the extension of SpRecA nucleopolymers. Instead, we found that the conserved RadA helicase promotes SpRecA nucleofilamentation in an ATP-dependent manner. This allowed us to solve the atomic structure of such a long native SpRecA nucleopolymer by cryoEM stabilized with ATP␥S. It was found to be equivalent to the crystal structure of the EcRecA filament with a marked difference in how RecA mediates nucleotide orientation in the stretched ssDNA. Then, our results show that SpRecA and EcRecA HR activities are different, in correlation with their distinct ATP-dependent ss-DNA binding modes. | |
| dc.language.iso | EN | en_US |
| dc.title.en | Assembly mechanism and cryoEM structure of RecA recombination nucleofilaments from Streptococcus pneumoniae | |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1093/nar/gkad080 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio] | en_US |
| dc.identifier.pubmed | 36806960 | en_US |
| bordeaux.journal | Nucleic Acids Research | en_US |
| bordeaux.page | 2800 - 2817 | en_US |
| bordeaux.volume | 51 | en_US |
| bordeaux.hal.laboratories | MFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234 | en_US |
| bordeaux.issue | 6 | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.import.source | hal | |
| hal.identifier | hal-04309334 | |
| hal.version | 1 | |
| hal.popular | non | en_US |
| hal.audience | Internationale | en_US |
| hal.export | false | |
| workflow.import.source | hal | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Nucleic%20Acids%20Research&rft.date=2023-02-21&rft.volume=51&rft.issue=6&rft.spage=2800%20-%202817&rft.epage=2800%20-%202817&rft.eissn=0305-1048&rft.issn=0305-1048&rft.au=HERTZOG,%20Maud&PERRY,%20Thomas&DUPAIGNE,%20Pauline&SERRES,%20Sandra&MORALES,%20Violette&rft.genre=article |
