Protein resonance assignment by solid-state NMR based on 1H-detected 13C double-quantum spectroscopy at fast MAS
| dc.rights.license | open | en_US |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | LENDS, Alons | |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | BERBON, Melanie | |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | HABENSTEIN, Birgit | |
| dc.contributor.author | NISHIYAMA, Yusuke | |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | LOQUET, Antoine | |
| dc.date.accessioned | 2024-04-16T13:41:34Z | |
| dc.date.available | 2024-04-16T13:41:34Z | |
| dc.date.issued | 2021-12-01 | |
| dc.identifier.issn | 1573-5001 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/199112 | |
| dc.description.abstractEn | Solid-state NMR spectroscopy is a powerful technique to study insoluble and non-crystalline proteins and protein complexes at atomic resolution. The development of proton (1H) detection at fast magic-angle spinning (MAS) has considerably increased the analytical capabilities of the technique, enabling the acquisition of 1H-detected fingerprint experiments in few hours. Here an approach based on double-quantum (DQ) 13C spectroscopy, detected on 1H, is proposed for fast MAS regime (> 60 kHz) to perform the sequential assignment of insoluble proteins of small size, without any specific deuteration requirement. By combining two three-dimensional 1H detected experiments correlating a 13C DQ dimension respectively to its intra-residue and sequential 15 N-1H pairs, a sequential walk through DQ (Ca + CO) resonance is obtained. The approach takes advantage of fast MAS to achieve an efficient sensitivity and the addition of a DQ dimension provides spectral features useful for the resonance assignment process. | |
| dc.language.iso | EN | en_US |
| dc.subject.en | Fast MAS | |
| dc.subject.en | Protein NMR | |
| dc.subject.en | Proton detection | |
| dc.subject.en | Solid-State NMR | |
| dc.title.en | Protein resonance assignment by solid-state NMR based on 1H-detected 13C double-quantum spectroscopy at fast MAS | |
| dc.title.alternative | J Biomol NMR | en_US |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1007/s10858-021-00386-6 | en_US |
| dc.subject.hal | Chimie/Matériaux | en_US |
| dc.identifier.pubmed | 34813018 | en_US |
| bordeaux.journal | Journal of biomolecular NMR | en_US |
| bordeaux.page | 417-427 | en_US |
| bordeaux.volume | 75 | en_US |
| bordeaux.hal.laboratories | CBMN : Chimie & de Biologie des Membranes & des Nano-objets - UMR 5248 | en_US |
| bordeaux.issue | 10 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | Bordeaux INP | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| hal.popular | non | en_US |
| hal.audience | Internationale | en_US |
| hal.export | false | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20biomolecular%20NMR&rft.date=2021-12-01&rft.volume=75&rft.issue=10&rft.spage=417-427&rft.epage=417-427&rft.eissn=1573-5001&rft.issn=1573-5001&rft.au=LENDS,%20Alons&BERBON,%20Melanie&HABENSTEIN,%20Birgit&NISHIYAMA,%20Yusuke&LOQUET,%20Antoine&rft.genre=article |
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