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Effects of Chain Length of Chitosan Oligosaccharides on Solution Properties and Complexation with siRNA

dc.rights.licenseopen
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorDELAS, Tim
hal.structure.identifierIngénierie des Matériaux Polymères [IMP]
dc.contributor.authorMOCK-JOUBERT, Maxime
hal.structure.identifierIngénierie des Matériaux Polymères [IMP]
dc.contributor.authorFAIVRE, Jimmy
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorHOFMAIER, Mirjam
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorSANDRE, Olivier
hal.structure.identifierCentre de Recherche Paul Pascal [CRPP]
dc.contributor.authorDOLE, Francois
hal.structure.identifierCentre de Recherche Paul Pascal [CRPP]
dc.contributor.authorCHAPEL, Jean
hal.structure.identifierIngénierie des Matériaux Polymères [IMP]
dc.contributor.authorCRÉPET, Agnès
hal.structure.identifierIngénierie des Matériaux Polymères [IMP]
dc.contributor.authorTROMBOTTO, Stéphane
hal.structure.identifierIngénierie des Matériaux Polymères [IMP]
dc.contributor.authorDELAIR, Thierry
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorSCHATZ, Christophe
dc.date.accessioned2020
dc.date.available2020
dc.date.issued2019
dc.identifier.issn2073-4360
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/19846
dc.description.abstractEnIn the context of gene delivery, chitosan has been widely used as a safe and effective polycation to complex DNA, RNA and more recently, siRNA. However, much less attention has been paid to chitosan oligosaccharides (COS) despite their biological properties. This study proposed to carry out a physicochemical study of COS varying in degree of polymerization (DP) from 5 to 50, both from the point of view of the solution properties and the complexing behavior with siRNA. The main parameters studied as a function of DP were the apparent pKa, the solubility versus pH, the binding affinity with siRNA and the colloidal properties of complexes. Some parameters, like the pKa or the binding enthalpy with siRNA, showed a marked transition from DP 5 to DP 13, suggesting that electrostatic properties of COS vary considerably in this range of DP. The colloidal properties of siRNA/COS complexes were affected in a different way by the COS chain length. In particular, COS of relatively high DP (≥50) were required to form small complex particles with good stability.
dc.description.sponsorshipMarquage non-covalent de siRNA par de petites séquences de copolymères à blocs pour une meilleure administration ciblée - ANR-16-CE09-0020
dc.language.isoen
dc.publisherMDPI
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subject.enelectrostatics
dc.subject.enpolyionic complexation
dc.subject.enchitosan
dc.subject.ensiRNA
dc.subject.enoligosaccharide
dc.subject.enpolyelectrolyte
dc.title.enEffects of Chain Length of Chitosan Oligosaccharides on Solution Properties and Complexation with siRNA
dc.typeArticle de revue
dc.identifier.doi10.3390/polym11081236
dc.subject.halChimie/Polymères
dc.subject.halPhysique [physics]/Matière Condensée [cond-mat]/Matière Molle [cond-mat.soft]
dc.subject.halSciences du Vivant [q-bio]/Biotechnologies
bordeaux.journalPolymers
bordeaux.page1236
bordeaux.volume11
bordeaux.hal.laboratoriesLaboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629*
bordeaux.issue8
bordeaux.institutionBordeaux INP
bordeaux.institutionUniversité de Bordeaux
bordeaux.peerReviewedoui
hal.identifierhal-02194615
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02194615v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Polymers&rft.date=2019&rft.volume=11&rft.issue=8&rft.spage=1236&rft.epage=1236&rft.eissn=2073-4360&rft.issn=2073-4360&rft.au=DELAS,%20Tim&MOCK-JOUBERT,%20Maxime&FAIVRE,%20Jimmy&HOFMAIER,%20Mirjam&SANDRE,%20Olivier&rft.genre=article


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