DELAS, Tim; MOCK-JOUBERT, Maxime; FAIVRE, Jimmy; HOFMAIER, Mirjam; SANDRE, Olivier; DOLE, Francois; CHAPEL, Jean; CRÉPET, Agnès; TROMBOTTO, Stéphane; DELAIR, Thierry; SCHATZ, Christophe

doi:10.3390/polym11081236

Métadonnées

Afficher la notice complète

Licence d’utilisation du document

DELAS, Tim
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences

MOCK-JOUBERT, Maxime
Ingénierie des Matériaux Polymères [IMP]

FAIVRE, Jimmy
Ingénierie des Matériaux Polymères [IMP]

Langue

Article de revue

Ce document a été publié dans

Polymers. 2019, vol. 11, n° 8, p. 1236

MDPI

Résumé en anglais

In the context of gene delivery, chitosan has been widely used as a safe and effective polycation to complex DNA, RNA and more recently, siRNA. However, much less attention has been paid to chitosan oligosaccharides (COS) despite their biological properties. This study proposed to carry out a physicochemical study of COS varying in degree of polymerization (DP) from 5 to 50, both from the point of view of the solution properties and the complexing behavior with siRNA. The main parameters studied as a function of DP were the apparent pKa, the solubility versus pH, the binding affinity with siRNA and the colloidal properties of complexes. Some parameters, like the pKa or the binding enthalpy with siRNA, showed a marked transition from DP 5 to DP 13, suggesting that electrostatic properties of COS vary considerably in this range of DP. The colloidal properties of siRNA/COS complexes were affected in a different way by the COS chain length. In particular, COS of relatively high DP (≥50) were required to form small complex particles with good stability.< Réduire

Mots clés en anglais

electrostatics

polyionic complexation

chitosan

siRNA

oligosaccharide

polyelectrolyte

URI

https://oskar-bordeaux.fr/handle/20.500.12278/19846

DOI

http://dx.doi.org/10.3390/polym11081236

Project ANR

Marquage non-covalent de siRNA par de petites séquences de copolymères à blocs pour une meilleure administration ciblée - ANR-16-CE09-0020

Origine

Importé de hal

Unités de recherche

Laboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629