Cellulose biosaccharification by Irpex lacteus wood decay fungus
BOIKO, Sergiy
National Academy of Sciences of Ukraine = Національна академія наук України = Académie nationale des sciences d'Ukraine [NASU / НАН України]
National Academy of Sciences of Ukraine = Національна академія наук України = Académie nationale des sciences d'Ukraine [NASU / НАН України]
NETSVETOV, Maksym
Biodiversité, Gènes & Communautés [BioGeCo]
National Academy of Sciences of Ukraine = Національна академія наук України = Académie nationale des sciences d'Ukraine [NASU / НАН України]
Biodiversité, Gènes & Communautés [BioGeCo]
National Academy of Sciences of Ukraine = Національна академія наук України = Académie nationale des sciences d'Ukraine [NASU / НАН України]
RADCHENKO, Vladimir
National Academy of Sciences of Ukraine = Національна академія наук України = Académie nationale des sciences d'Ukraine [NASU / НАН України]
National Academy of Sciences of Ukraine = Національна академія наук України = Académie nationale des sciences d'Ukraine [NASU / НАН України]
BOIKO, Sergiy
National Academy of Sciences of Ukraine = Національна академія наук України = Académie nationale des sciences d'Ukraine [NASU / НАН України]
National Academy of Sciences of Ukraine = Національна академія наук України = Académie nationale des sciences d'Ukraine [NASU / НАН України]
NETSVETOV, Maksym
Biodiversité, Gènes & Communautés [BioGeCo]
National Academy of Sciences of Ukraine = Національна академія наук України = Académie nationale des sciences d'Ukraine [NASU / НАН України]
Biodiversité, Gènes & Communautés [BioGeCo]
National Academy of Sciences of Ukraine = Національна академія наук України = Académie nationale des sciences d'Ukraine [NASU / НАН України]
RADCHENKO, Vladimir
National Academy of Sciences of Ukraine = Національна академія наук України = Académie nationale des sciences d'Ukraine [NASU / НАН України]
< Réduire
National Academy of Sciences of Ukraine = Національна академія наук України = Académie nationale des sciences d'Ukraine [NASU / НАН України]
Langue
en
Article de revue
Ce document a été publié dans
MADERAS: Ciencia y Tecnología. 2023-05, vol. 25
Universidad del Bío-Bío
Résumé en anglais
Enzymatic hydrolysis is an environmentally friendly technology to produce sugars from pretreated biomass. Here, we show that the new Il-11 Irpex lacteus strain can synthesize cellulases in a high quantity. The peptone and ...Lire la suite >
Enzymatic hydrolysis is an environmentally friendly technology to produce sugars from pretreated biomass. Here, we show that the new Il-11 Irpex lacteus strain can synthesize cellulases in a high quantity. The peptone and filter paper contained in the medium significantly enhanced activity of endo-1,4-fi-D-glucanases (app. 50 IU/mL) and total cellulases (app. 9 IU/mL), whereas the medium with peptone and sodium carboxymethyl cellulose stimulated activity of exo-1,4-fi-D-glucanases (33 IU/mL). The expression of cellulases reached its maximum within 96-144 hours, and the optimum pH is 3,7. Thermal treatment at 30 & DEG;C for 60 minutes activated endo-1,4-fi-D-glucanases and total cellulases, while exo-1,4-fi-D-glucanases activity was enhanced following 40 & DEG;C treatment. In total, the cellulases complex (300 IU/g) saccharified untreated cellulose by 38 % in 48 hours. Concentrate with filter paper activity 100 IU/g is the more balanced enzyme-substrate ratio (2 %), which allows prolonging the saccharification process that will have a positive effect on the cost of the final product.< Réduire
Mots clés en anglais
Cellulose
endo-1
4-β-D-glucanases
exo-1
Irpex lacteus
saccharification
thermostability
Origine
Importé de halUnités de recherche