Dynamic Spatial Formation and Distribution of Intrinsically Disordered Protein Droplets in Macromolecularly Crowded Protocells
dc.rights.license | open | |
hal.structure.identifier | Laboratoire de Chimie des Polymères Organiques [LCPO] | |
hal.structure.identifier | Team 3 LCPO : Polymer Self-Assembly & Life Sciences | |
dc.contributor.author | ZHAO, Hang | |
hal.structure.identifier | Laboratoire de Chimie des Polymères Organiques [LCPO] | |
hal.structure.identifier | Team 3 LCPO : Polymer Self-Assembly & Life Sciences | |
dc.contributor.author | IBRAHIMOVA, Vusala | |
hal.structure.identifier | Laboratoire de Chimie des Polymères Organiques [LCPO] | |
hal.structure.identifier | Team 3 LCPO : Polymer Self-Assembly & Life Sciences | |
dc.contributor.author | GARANGER, Elisabeth
IDREF: 089451740 | |
hal.structure.identifier | Laboratoire de Chimie des Polymères Organiques [LCPO] | |
hal.structure.identifier | Team 3 LCPO : Polymer Self-Assembly & Life Sciences | |
dc.contributor.author | LECOMMANDOUX, Sebastien | |
dc.date.accessioned | 2020 | |
dc.date.available | 2020 | |
dc.date.issued | 2020 | |
dc.identifier.issn | 1433-7851 | |
dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/19698 | |
dc.description.abstractEn | Elastin‐like polypeptides (ELPs) have been proposed as a simple model of intrinsically disordered proteins (IDPs) which can form membrane‐less organelles via liquid‐liquid phase separation (LLPS) in cellular milieu. Herein, fluorescently labeled ELP is studied in cytomimetic aqueous two‐phase system (ATPS). Droplet‐based protocells are obtained in a microfluidic system, allowing for confinement, temperature changes and statistical analysis. The spatial organization of ELP is observed in such binary ATPS macrocrowders. In addition, owing to switch of conformational states, dynamic formation and distribution of ELP‐rich droplets within the artificial cytoplasm is triggered by temperature. Three‐dimensional structured proteins are concurrently encapsulated along with ELP in synthetic cells and distinct partitioning properties of these proteins and ELP in binary polymeric phases are observed. This underpinning discovery demonstrates that the ability of ELP to coacervate with temperature can be maintained inside intracellular mimetic medium, and the preferential distribution of ELP in macromolecular crowding. | |
dc.description.sponsorship | IdEx Bordeaux - ANR-10-IDEX-0003-02/10-IDEX-0003 | |
dc.language.iso | en | |
dc.publisher | Wiley-VCH Verlag | |
dc.subject.en | Intrinsically disordered proteins (IDP) | |
dc.subject.en | Elastin‐like polypeptides (ELP) | |
dc.subject.en | synthetic cell | |
dc.subject.en | minimal cell | |
dc.subject.en | macromolecular crowding | |
dc.subject.en | aqueous two‐phase system (ATPS) | |
dc.title.en | Dynamic Spatial Formation and Distribution of Intrinsically Disordered Protein Droplets in Macromolecularly Crowded Protocells | |
dc.type | Article de revue | |
dc.identifier.doi | 10.1002/anie.202001868 | |
dc.subject.hal | Physique [physics]/Matière Condensée [cond-mat]/Matière Molle [cond-mat.soft] | |
dc.subject.hal | Chimie/Polymères | |
dc.subject.hal | Sciences du Vivant [q-bio]/Biotechnologies | |
dc.description.sponsorshipEurope | Temperature-responsive polypeptide nanocarriers for abdominal therapies | |
bordeaux.journal | Angewandte Chemie International Edition | |
bordeaux.page | 11028-11036 | |
bordeaux.volume | 59 | |
bordeaux.hal.laboratories | Laboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629 | * |
bordeaux.issue | 27 | |
bordeaux.institution | Bordeaux INP | |
bordeaux.institution | Université de Bordeaux | |
bordeaux.peerReviewed | oui | |
hal.identifier | hal-02519909 | |
hal.version | 1 | |
hal.origin.link | https://hal.archives-ouvertes.fr//hal-02519909v1 | |
bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Angewandte%20Chemie%20International%20Edition&rft.date=2020&rft.volume=59&rft.issue=27&rft.spage=11028-11036&rft.epage=11028-11036&rft.eissn=1433-7851&rft.issn=1433-7851&rft.au=ZHAO,%20Hang&IBRAHIMOVA,%20Vusala&GARANGER,%20Elisabeth&LECOMMANDOUX,%20Sebastien&rft.genre=article |
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