Mostrar el registro sencillo del ítem

Surface Immobilized Nucleic Acid–Transcription Factor Quantum Dots for Biosensing

dc.rights.licenseopen
hal.structure.identifierBoston University [Boston] [BU]
hal.structure.identifierDepartment of Biomedical Engineering [Boston]
dc.contributor.authorCHEN, Mingfu
hal.structure.identifierBoston University [Boston] [BU]
hal.structure.identifierDepartment of Biomedical Engineering [Boston]
dc.contributor.authorNGUYEN, Thuy T.
hal.structure.identifierBoston University [Boston] [BU]
hal.structure.identifierDepartment of Biomedical Engineering [Boston]
dc.contributor.authorVARONGCHAYAKUL, Nitinun
hal.structure.identifierBoston University [Boston] [BU]
hal.structure.identifierDepartment of Chemistry and Chemical Biology [Boston]
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorGRAZON, Chloé
hal.structure.identifierBoston University [Boston] [BU]
hal.structure.identifierDepartment of Biomedical Engineering [Boston]
dc.contributor.authorCHERN, Margaret
hal.structure.identifierBoston University [Boston] [BU]
hal.structure.identifierNational Emerging Infectious Diseases Laboratories [NEIDL]
dc.contributor.authorBAER, R. C.
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorLECOMMANDOUX, Sebastien
hal.structure.identifierBoston University [Boston] [BU]
hal.structure.identifierDepartment of Biomedical Engineering [Boston]
dc.contributor.authorKLAPPERICH, Catherine M.
hal.structure.identifierBoston University [Boston] [BU]
hal.structure.identifierDepartment of Biomedical Engineering [Boston]
dc.contributor.authorGALAGAN, James E.
hal.structure.identifierBoston University [Boston] [BU]
hal.structure.identifierDepartment of Biomedical Engineering [Boston]
dc.contributor.authorDENNIS, Allison M.
hal.structure.identifierBoston University [Boston] [BU]
hal.structure.identifierDepartment of Chemistry and Chemical Biology [Boston]
dc.contributor.authorGRINSTAFF, Mark W.
dc.date2020
dc.date.accessioned2020
dc.date.available2020
dc.date.issued2020
dc.identifier.issn2192-2640
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/19551
dc.description.abstractEnImmobilization of biosensors on surfaces is a key step towards development of devices for real world applications. Here we describe the preparation, characterization, and evaluation of a surface bound transcription factor – nucleic acid complex for analyte detection as an alternative to conventional systems employing aptamers or antibodies. The sensor consists of a gold surface modified with thiolated Cy5 fluorophore-labeled DNA and an allosteric transcription factor (TetR) linked to a quantum dot. Upon addition of anhydrotetracycline (aTc) – the analyte – the TetR-QDs release from the surface-bound DNA, resulting in loss of the Förster resonance energy transfer (FRET) signal. The sensor responds in a dose-dependent manner over the relevant range of 0-200 µM aTc with a limit of detection of 80 nM. The fabrication of the sensor and the subsequent real-time quantitative measurements establish a framework for the design of future surface-bound, affinity-based biosensors using allosteric transcription factors for molecular recognition.
dc.language.isoen
dc.publisherWiley
dc.subject.enbiosensing
dc.subject.entranscription factor
dc.subject.enquantum dots
dc.subject.enFörster resonance energy transfer
dc.title.enSurface Immobilized Nucleic Acid–Transcription Factor Quantum Dots for Biosensing
dc.typeArticle de revue
dc.subject.halChimie/Matériaux
dc.description.sponsorshipEuropeA Wearable Sensor for Hormones Based on a Native Microbial Sensing
bordeaux.journalAdvanced Healthcare Materials
bordeaux.page2000403
bordeaux.volume9
bordeaux.hal.laboratoriesLaboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629*
bordeaux.issue17
bordeaux.institutionBordeaux INP
bordeaux.institutionUniversité de Bordeaux
bordeaux.peerReviewedoui
hal.identifierhal-02991798
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02991798v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Advanced%20Healthcare%20Materials&rft.date=2020&rft.volume=9&rft.issue=17&rft.spage=2000403&rft.epage=2000403&rft.eissn=2192-2640&rft.issn=2192-2640&rft.au=CHEN,%20Mingfu&NGUYEN,%20Thuy%20T.&VARONGCHAYAKUL,%20Nitinun&GRAZON,%20Chlo%C3%A9&CHERN,%20Margaret&rft.genre=article


Archivos en el ítem

ArchivosTamañoFormatoVer

No hay archivos asociados a este ítem.

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem