BEAUDIER, Pierre; VILOTTE, Florent; SIMON, Marina; MUGGIOLU, Giovanna; LE TREQUESSER, Quentin; DEVÈS, Guillaume; PLAWINSKI, Laurent; MIKAEL, Antoine; CARON, Jérôme; KANTOR, Guy; DUPUY, Denis; DELVILLE, Marie-Hélène; BARBERET, Philippe; SEZNEC, Hervé

doi:10.1038/s41598-024-53847-x

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http://creativecommons.org/licenses/by-nc-nd/

BEAUDIER, Pierre
Laboratoire de Physique des Deux Infinis Bordeaux [LP2I - Bordeaux]
Institut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
Institut Européen de Chimie et Biologie [IECB]

VILOTTE, Florent
Laboratoire de Physique des Deux Infinis Bordeaux [LP2I - Bordeaux]
Institut Bergonié [Bordeaux]

SIMON, Marina
Laboratoire de Physique des Deux Infinis Bordeaux [LP2I - Bordeaux]
Institut Bergonié [Bordeaux]

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Article de revue

Ce document a été publié dans

Scientific Reports. 2024, vol. 14, p. 3295

Nature Publishing Group

Résumé en anglais

This study aimed to explore the potential of metal oxides such as Titanate Scrolled Nanosheets (TNs) in improving the radiosensitivity of sarcoma cell lines. Enhancing the response of cancer cells to radiation therapy is crucial, and one promising approach involves utilizing metal oxide nanoparticles. We focused on the impact of exposing two human sarcoma cell lines to both TNs and ionizing radiation (IR). Our research was prompted by previous in vitro toxicity assessments, revealing a correlation between TNs' toxicity and alterations in intracellular calcium homeostasis. A hydrothermal process using titanium dioxide powder in an alkaline solution produced the TNs. Our study quantified the intracellular content of TNs and analyzed their impact on radiation-induced responses. This assessment encompassed PIXE analysis, cell proliferation, and transcriptomic analysis. We observed that sarcoma cells internalized TNs, causing alterations in intracellular calcium homeostasis. We also found that irradiation influence intracellular calcium levels. Transcriptomic analysis revealed marked disparities in the gene expression patterns between the two sarcoma cell lines, suggesting a potential cell-line-dependent nano-sensitization to IR. These results significantly advance our comprehension of the interplay between TNs, IR, and cancer cells, promising potential enhancement of radiation therapy efficiency.< Réduire

DOI

http://dx.doi.org/10.1038/s41598-024-53847-x

Projet Européen

Support of Public and Industrial Research using Ion Beam Technology
Supporting Postgraduate Research with Internships in industry and Training Excellence

Project ANR

Mécanismes d'internalisation et de toxicité des nanoparticules d'oxyde de titane dans des organismes multicellulaires eucaryotes

Origine

Importé de hal

Unités de recherche

Institut de Chimie de la Matière Condensée de Bordeaux (ICMCB) - UMR 5026