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Serotonergic neurons are involved in the counter-regulatory response to hypoglycemia

dc.rights.licenseopenen_US
hal.structure.identifierNutrition et Neurobiologie intégrée [NutriNeuro]
dc.contributor.authorMARTIN, Hugo
hal.structure.identifierNutrition et Neurobiologie intégrée [NutriNeuro]
dc.contributor.authorCOURSAN, Adeline
hal.structure.identifierUnité de Biologie Fonctionnelle et Adaptative [BFA (UMR_8251 / U1133)]
dc.contributor.authorLALLEMENT, Justine
dc.contributor.authorDI MICELI, Mathieu
hal.structure.identifierUnité de Biologie Fonctionnelle et Adaptative [BFA (UMR_8251 / U1133)]
dc.contributor.authorKANDIAH, Janany
hal.structure.identifierUnité de Biologie Fonctionnelle et Adaptative [BFA (UMR_8251 / U1133)]
dc.contributor.authorRAHO, Ilyes
hal.structure.identifierInstitut de Neurosciences cognitives et intégratives d'Aquitaine [INCIA]
dc.contributor.authorBUTTLER, Jasmine
hal.structure.identifierCentre de recherche en épidémiologie et santé des populations [CESP]
dc.contributor.authorGUILLOUX, Jean Philippe
hal.structure.identifierInstitut de Neurosciences cognitives et intégratives d'Aquitaine [INCIA]
dc.contributor.authorDE DEURWAERDERE, Philippe D.
hal.structure.identifierNutrition et Neurobiologie intégrée [NutriNeuro]
dc.contributor.authorLAYE, Sophie
ORCID: 0000-0002-3843-1012
IDREF: 11366883X
dc.contributor.authorROUTH, Vanessa H.
hal.structure.identifierCentre de Recherches sur la Cognition Animale - UMR5169 [CRCA]
dc.contributor.authorGUIARD, Bruno
hal.structure.identifierUnité de Biologie Fonctionnelle et Adaptative [BFA (UMR_8251 / U1133)]
dc.contributor.authorMAGNAN, Chr̀istophe N.
hal.structure.identifierUnité de Biologie Fonctionnelle et Adaptative [BFA (UMR_8251 / U1133)]
dc.contributor.authorCRUCIANI-GUGLIELMACCI, Celine
hal.structure.identifierNutrition et Neurobiologie intégrée [NutriNeuro]
dc.contributor.authorFIORAMONTI, Xavier
dc.date.accessioned2024-01-15T13:10:09Z
dc.date.available2024-01-15T13:10:09Z
dc.date.issued2023-10
dc.identifier.issn0953-8194en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/187169
dc.description.abstractEnObjectives: Intensive insulin therapy provides optimal glycemic control in patients with diabetes. However, intensive insulin therapy causes so-called iatrogenic hypoglycemia as a major adverse effect. The ventromedial hypothalamus (VMH) has been described as the primary brain area initiating the counter-regulatory response (CRR). Nevertheless, the VMH receives projections from other brain areas which could participate in the regulation of the CRR. In particular, studies suggest a potential role of the serotonin (5-HT) network. Thus, the objective of this study was to determine the contribution of 5-HT neurons in CRR control. Methods: Complementary approaches have been used to test this hypothesis in quantifying the level of 5-HT in several brain areas by HPLC in response to insulin-induced hypoglycemia, measuring the electrical activity of dorsal raphe (DR) 5-HT neurons in response to insulin or decreased glucose level by patch-clamp electrophysiology; and measuring the CRR hormone glucagon as an index of the CRR to the modulation of the activity of 5-HT neurons using pharmacological or pharmacogenetic approaches. Results: HPLC measurements show that the 5HIAA/5HT ratio is increased in several brain regions including the VMH in response to insulin-induced hypoglycemia. Patch-clamp electrophysiological recordings show that insulin, but not decreased glucose level, increases the firing frequency of DR 5-HT neurons in the DR. In vivo, both the pharmacological inhibition of 5-HT neurons by intraperitoneal injection of the 5-HT1A receptor agonist 8-OH-DPAT or the chemogenetic inhibition of these neurons reduce glucagon secretion, suggesting an impaired CRR. Conclusion: Taken together, these data highlight a new neuronal network involved in the regulation of the CRR. In particular, this study shows that DR 5-HT neurons detect iatrogenic hypoglycemia in response to the increased insulin level and may play an important role in the regulation of CRR.
dc.language.isoENen_US
dc.subject.enchemogenetic
dc.subject.englucagon
dc.subject.enhypoglycemia
dc.subject.eninsulin
dc.subject.enserotonin
dc.title.enSerotonergic neurons are involved in the counter-regulatory response to hypoglycemia
dc.typeArticle de revueen_US
dc.identifier.doi10.1111/jne.13344en_US
dc.subject.halSciences du Vivant [q-bio]/Neurosciences [q-bio.NC]en_US
dc.identifier.pubmed37857383en_US
bordeaux.journalJournal of Neuroendocrinologyen_US
bordeaux.volume35en_US
bordeaux.hal.laboratoriesNutriNeuro (Laboratoire de Nutrition et Neurobiologie Intégrée) - UMR 1286en_US
bordeaux.issue12en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionINRAEen_US
bordeaux.institutionCNRS
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.identifier.funderIDFondation pour la Recherche Médicaleen_US
bordeaux.identifier.funderIDInstitut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnementen_US
bordeaux.import.sourcehal
hal.identifierhal-04342557
hal.version1
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
workflow.import.sourcehal
dc.rights.ccPas de Licence CCen_US
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