DECROS, Guillaume; DUSSARRAT, Thomas; BALDET, Pierre; CASSAN, Cédric; CABASSON, Cécile; DIEUAIDE‐NOUBHANI, Martine; DESTAILLEUR, Alice; FLANDIN, Amélie; PRIGENT, Sylvain; MORI, Kentaro; COLOMBIE, Sophie; JORLY, Joana; GIBON, Yves; BEAUVOIT, Bertrand; PÉTRIACQ, Pierre

doi:10.1111/nph.19160

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http://creativecommons.org/licenses/by-nc/

DECROS, Guillaume
Biologie du fruit et pathologie [BFP]

DUSSARRAT, Thomas
Biologie du fruit et pathologie [BFP]

BALDET, Pierre
Biologie du fruit et pathologie [BFP]

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Article de revue

Ce document a été publié dans

New Phytologist. 2023-08-07

Wiley

Résumé en anglais

Summary The ascorbate–glutathione (ASC–GSH) cycle is at the heart of redox metabolism, linking the major redox buffers with central metabolism through the processing of reactive oxygen species (ROS) and pyridine nucleotide metabolism. Tomato fruit development is underpinned by changes in redox buffer contents and their associated enzyme capacities, but interactions between them remain unclear. Based on quantitative data obtained for the core redox metabolism, we built an enzyme‐based kinetic model to calculate redox metabolite concentrations with their corresponding fluxes and control coefficients. Dynamic and associated regulations of the ASC–GSH cycle throughout the whole fruit development were analysed and pointed to a sequential metabolic control of redox fluxes by ASC synthesis, NAD(P)H and ROS availability depending on the developmental phase. Furthermore, we highlighted that monodehydroascorbate reductase and the availability of reducing power were found to be the main regulators of the redox state of ASC and GSH during fruit growth under optimal conditions. Our kinetic modelling approach indicated that tomato fruit development displayed growth phase‐dependent redox metabolism linked with central metabolism via pyridine nucleotides and H 2 O 2 availability, while providing a new tool to the scientific community to investigate redox metabolism in fruits.< Réduire

Mots clés en anglais

ASC-GSH cycle

enzyme-based kinetic modelling

fruit development

metabolism

redox

tomato

DOI

http://dx.doi.org/10.1111/nph.19160

Projet Européen

European Commission’s Horizon 2020 Research and Innovation program via the GLOMICAVE project under grant agreement no. 952908

Project ANR

Modélisation intégrative du fruit pour un système de sélection unifié - ANR-15-CE20-0009
Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation
Centre français de phénomique végétale

Origine

Importé de hal

Unités de recherche

Biologie du Fruit & Pathologie (BFP) - UMR 1332