Diversity and pathobiology of an ilarvirus unexpectedly detected in diverse plants and global sequencing data
RIVAREZ, Mark Paul Selda
National Institute of Biology [Ljubljana] [NIB]
North Carolina State University [Raleigh] [NC State]
Caraga State University
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National Institute of Biology [Ljubljana] [NIB]
North Carolina State University [Raleigh] [NC State]
Caraga State University
RIVAREZ, Mark Paul Selda
National Institute of Biology [Ljubljana] [NIB]
North Carolina State University [Raleigh] [NC State]
Caraga State University
< Leer menos
National Institute of Biology [Ljubljana] [NIB]
North Carolina State University [Raleigh] [NC State]
Caraga State University
Idioma
en
Article de revue
Este ítem está publicado en
Phytopathology. 2023, vol. 113, n° 9, p. 1729-1744
American Phytopathological Society
Resumen en inglés
High-throughput sequencing (HTS) and sequence mining tools revolutionized virus detection and discovery in recent years and implementing them with classical plant virology techniques results to a powerful approach to ...Leer más >
High-throughput sequencing (HTS) and sequence mining tools revolutionized virus detection and discovery in recent years and implementing them with classical plant virology techniques results to a powerful approach to characterize viruses. An example of a virus discovered through HTS is Solanum nigrum ilarvirus 1 (SnIV1) (Bromoviridae), which was recently reported in various solanaceous plants from France, Slovenia, Greece, and South Africa. It was likewise detected in grapevines (Vitaceae) and several Fabaceae and Rosaceae plant species. Such very diverse set of source organisms is atypical for ilarviruses, thus warranting further investigation. In this study, modern and classical virological tools were combined to accelerate the characterization of SnIV1. Through HTS-based virome surveys, mining of sequence read archive datasets, and literature search, SnIV1 was further identified from diverse plant and non-plant sources globally. SnIV1 isolates showed relatively low variability compared to other phylogenetically related ilarviruses. Phylogenetic analyses showed a distinct basal clade of isolates from Europe, while the rest formed clades of mixed geographic origin. Furthermore, systemic infection of SnIV1 in Solanum villosum and its mechanical and graft transmissibility to solanaceous species were demonstrated. Near identical SnIV1 genomes from the inoculum (S. villosum) and inoculated Nicotiana benthamiana were sequenced, thus partially fulfilling Koch’s postulates. SnIV1 was shown to be seed-transmitted and potentially pollen-borne, has spherical virions, and possibly induces histopathological changes in infected N. benthamiana leaf tissues. Overall, this study provides information to better understand the diversity, global presence, and pathobiology of SnIV1, however, its possible emergence as a destructive pathogen remains uncertain.< Leer menos
Palabras clave en inglés
Ilarvirus
Solanaceae
Serratus
virus diversity
phylogenetics
histopathology
virion morphology
symptomatology
virus transmission
pollen
Proyecto europeo
INEXTVIR GA 813542
Orígen
Importado de HalCentros de investigación