Single α-particle irradiation permits real-time visualization of RNF8 accumulation at DNA damaged sites
| hal.structure.identifier | Centre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG] | |
| dc.contributor.author | MUGGIOLU, Giovanna | |
| hal.structure.identifier | Laboratoire Capteurs Diamant (CEA, LIST) [LCD (CEA, LIST)] | |
| dc.contributor.author | POMORSKI, Michal | |
| hal.structure.identifier | Centre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG] | |
| dc.contributor.author | CLAVERIE, Gérard | |
| hal.structure.identifier | Laboratoire Capteurs Diamant (CEA, LIST) [LCD (CEA, LIST)] | |
| dc.contributor.author | BERTHET, Guillaume | |
| hal.structure.identifier | Laboratoire Capteurs Diamant (CEA, LIST) [LCD (CEA, LIST)] | |
| dc.contributor.author | MER-CALFATI, Christine | |
| hal.structure.identifier | Laboratoire Capteurs Diamant (CEA, LIST) [LCD (CEA, LIST)] | |
| dc.contributor.author | SAADA, Samuel | |
| hal.structure.identifier | Centre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG] | |
| dc.contributor.author | DEVÈS, Guillaume | |
| hal.structure.identifier | Centre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG] | |
| dc.contributor.author | SIMON, Marina | |
| hal.structure.identifier | Centre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG] | |
| dc.contributor.author | SEZNEC, Hervé | |
| hal.structure.identifier | Centre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG] | |
| dc.contributor.author | BARBERET, Philippe | |
| dc.date.accessioned | 2023-11-20T16:39:28Z | |
| dc.date.available | 2023-11-20T16:39:28Z | |
| dc.date.issued | 2017 | |
| dc.identifier.issn | 2045-2322 | |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/184957 | |
| dc.description.abstractEn | As well as being a significant source of environmental radiation exposure, α-particles are increasingly considered for use in targeted radiation therapy. A better understanding of α-particle induced damage at the DNA scale can be achieved by following their tracks in real-time in targeted living cells. Focused α-particle microbeams can facilitate this but, due to their low energy (up to a few MeV) and limited range, α-particles detection, delivery, and follow-up observations of radiation-induced damage remain difficult. In this study, we developed a thin Boron-doped Nano-Crystalline Diamond membrane that allows reliable single α-particles detection and single cell irradiation with negligible beam scattering. The radiation-induced responses of single 3 MeV α-particles delivered with focused microbeam are visualized in situ over thirty minutes after irradiation by the accumulation of the GFP-tagged RNF8 protein at DNA damaged sites. | |
| dc.language.iso | en | |
| dc.publisher | Nature Publishing Group | |
| dc.subject.en | instrumentation | |
| dc.subject.en | nuclear instrumentation | |
| dc.subject.en | ionizing radiation | |
| dc.subject.en | radioactivity | |
| dc.subject.en | alpha-rays | |
| dc.subject.en | radiotherapy | |
| dc.subject.en | alpha particles | |
| dc.subject.en | radiation-induced damage | |
| dc.subject.en | diamond | |
| dc.subject.en | boron doping | |
| dc.subject.en | nano-crystalline diamond | |
| dc.title.en | Single α-particle irradiation permits real-time visualization of RNF8 accumulation at DNA damaged sites | |
| dc.type | Article de revue | |
| dc.identifier.doi | 10.1038/srep41764 | |
| dc.subject.hal | Physique [physics]/Physique [physics]/Instrumentations et Détecteurs [physics.ins-det] | |
| dc.subject.hal | Physique [physics]/Physique [physics]/Physique Médicale [physics.med-ph] | |
| bordeaux.journal | Scientific Reports | |
| bordeaux.page | 41764 | |
| bordeaux.volume | 7 | |
| bordeaux.hal.laboratories | Centre d'Études Nucléaires de Bordeaux Gradignan (CENBG) | * |
| bordeaux.issue | 1 | |
| bordeaux.institution | Université de Bordeaux | |
| bordeaux.institution | CNRS | |
| bordeaux.peerReviewed | oui | |
| hal.identifier | hal-04146052 | |
| hal.version | 1 | |
| hal.popular | non | |
| hal.audience | Internationale | |
| hal.origin.link | https://hal.archives-ouvertes.fr//hal-04146052v1 | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Scientific%20Reports&rft.date=2017&rft.volume=7&rft.issue=1&rft.spage=41764&rft.epage=41764&rft.eissn=2045-2322&rft.issn=2045-2322&rft.au=MUGGIOLU,%20Giovanna&POMORSKI,%20Michal&CLAVERIE,%20G%C3%A9rard&BERTHET,%20Guillaume&MER-CALFATI,%20Christine&rft.genre=article |
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