De novo RNA synthesis catalyzed by the Zika Virus RNA polymerase domain.
| dc.rights.license | open | en_US |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | CALMELS, Christina | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | VENTURA, Michel | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | AKNIN, Cindy | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | METIFIOT, Mathieu | |
| dc.contributor.author | ANDREOLA, Marie-Line | |
| dc.date.accessioned | 2023-11-08T09:30:07Z | |
| dc.date.available | 2023-11-08T09:30:07Z | |
| dc.date.issued | 2017-06-02 | |
| dc.identifier.issn | 2045-2322 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/184677 | |
| dc.description.abstractEn | Mosquito- and tick-borne pathogens including Chikungunya, Dengue, Japanese encephalitis, West Nile, Yellow fever and Zika virus, represent a new economic and public health challenge. In the absence of effective vaccines and specific therapies, only supportive regimens are administrated for most of these infections. Thus, the development of a targeted therapy is mandatory to stop the rapid progression of these pathogens and preoccupant associated burdens such as Guillain-Barre syndrome, microcephaly. For this, it is essential to develop biochemical tools to help study and target key viral enzymes involved in replication such as helicase complexes, methyl-transferases and RNA-dependent RNA polymerases. Here, we show that a highly purified ZIKV polymerase domain is active in vitro. Importantly, we show that this isolated domain is capable of de novo synthesis of the viral genome and efficient elongation without terminal nucleotide transferase activity. Altogether, this isolated polymerase domain will be a precious tool to screen and optimize specific nucleoside and non-nucleoside inhibitors to fight against Zika infections. | |
| dc.language.iso | EN | en_US |
| dc.rights | Attribution 3.0 United States | * |
| dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | * |
| dc.subject.en | Catalysis | |
| dc.subject.en | Humans | |
| dc.subject.en | Models | |
| dc.subject.en | Molecular | |
| dc.subject.en | Protein Binding | |
| dc.subject.en | Protein Conformation | |
| dc.subject.en | Protein Domains | |
| dc.subject.en | Protein Interaction Domains and Motifs | |
| dc.subject.en | RNA | |
| dc.subject.en | Viral | |
| dc.subject.en | RNA-Dependent RNA Polymerase | |
| dc.subject.en | Transcription | |
| dc.subject.en | Genetic | |
| dc.subject.en | Virus Replication | |
| dc.subject.en | Zika Virus | |
| dc.subject.en | Zika Virus Infection | |
| dc.title.en | De novo RNA synthesis catalyzed by the Zika Virus RNA polymerase domain. | |
| dc.title.alternative | Sci Rep | en_US |
| dc.type | Article de revue | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Microbiologie et Parasitologie | en_US |
| dc.identifier.pubmed | 28577343 | en_US |
| bordeaux.journal | Scientific Reports | en_US |
| bordeaux.page | 2697 | en_US |
| bordeaux.volume | 7 | en_US |
| bordeaux.hal.laboratories | MFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234 | en_US |
| bordeaux.issue | 1 | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.import.source | pubmed | |
| hal.identifier | hal-04274777 | |
| hal.version | 1 | |
| hal.date.transferred | 2023-11-08T09:30:09Z | |
| hal.popular | non | en_US |
| hal.audience | Internationale | en_US |
| hal.export | true | |
| workflow.import.source | pubmed | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Scientific%20Reports&rft.date=2017-06-02&rft.volume=7&rft.issue=1&rft.spage=2697&rft.epage=2697&rft.eissn=2045-2322&rft.issn=2045-2322&rft.au=CALMELS,%20Christina&VENTURA,%20Michel&AKNIN,%20Cindy&METIFIOT,%20Mathieu&ANDREOLA,%20Marie-Line&rft.genre=article |
