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Expression of viral polymerase and phosphorylation of core protein determine core and capsid localization of the human hepatitis B virus

hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorDEROUBAIX, Aurélie
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorOSSEMAN, Quentin
dc.contributor.authorCASSANY, Aurélia
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorBEGU, Dominique
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorRAGUES, Jessica
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorKASSAB, Somar
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
hal.structure.identifierCentre d’études d’Agents Pathogènes et Biotechologies pour la Santé [CPBS]
dc.contributor.authorLAINÉ, Sébastien
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorKANN, Michael
dc.date.accessioned2023-07-18T08:01:43Z
dc.date.available2023-07-18T08:01:43Z
dc.date.issued2015-01-01
dc.identifier.issn0022-1317
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/183417
dc.description.abstractEnBiopsies from patients show that hepadnaviral core proteins and capsids-collectively called core-are found in the nucleus and cytoplasm of infected hepatocytes. In the majority of studies, cytoplasmic core localization is related to low viraemia while nuclear core localization is associated with high viral loads. In order to better understand the molecular interactions leading to core localization, we analysed transfected hepatoma cells using immune fluorescence microscopy. We observed that expression of core protein in the absence of other viral proteins led to nuclear localization of core protein and capsids, while expression of core in the context of the other viral proteins resulted in a predominantly cytoplasmic localization. Analysis of which viral partner was responsible for cytoplasmic retention indicated that the HBx, surface proteins and HBeAg had no impact but that the viral polymerase was the major determinant. Further analysis revealed that e, an RNA structure to which the viral polymerase binds, was essential for cytoplasmic retention. Furthermore, we showed that core protein phosphorylation at Ser 164 was essential for the cytoplasmic core localization phenotype, which is likely to explain differences observed between individual cells.
dc.language.isoen
dc.publisherMicrobiology Society
dc.title.enExpression of viral polymerase and phosphorylation of core protein determine core and capsid localization of the human hepatitis B virus
dc.typeArticle de revueen_US
dc.identifier.doi10.1099/vir.0.064816-0
dc.subject.halSciences du Vivant [q-bio]
bordeaux.page183 - 195
bordeaux.volume96
bordeaux.hal.laboratoriesMFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234en_US
bordeaux.issue1
bordeaux.institutionCNRS
bordeaux.peerReviewedoui
bordeaux.import.sourcehal
hal.identifierhal-03048123
hal.version1
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
workflow.import.sourcehal
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.date=2015-01-01&rft.volume=96&rft.issue=1&rft.spage=183%20-%20195&rft.epage=183%20-%20195&rft.eissn=0022-1317&rft.issn=0022-1317&rft.au=DEROUBAIX,%20Aur%C3%A9lie&OSSEMAN,%20Quentin&CASSANY,%20Aur%C3%A9lia&BEGU,%20Dominique&RAGUES,%20Jessica&rft.genre=article


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