Modulation of the functional association between the HIV-1 intasome and the nucleosome by histone amino-terminal tails
| dc.rights.license | open | en_US |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | BENLEULMI, Mohamed | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | MATYSIAK, Julien | |
| dc.contributor.author | ROBERT, Xavier | |
| dc.contributor.author | MISKEY, Csaba | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | MAURO, Eric | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | LAPAILLERIE, Delphine | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | LESBATS, Paul | |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | CHAIGNEPAIN, Stéphane | |
| dc.contributor.author | HENRIQUEZ, Daniel | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | CALMELS, Christina | |
| dc.contributor.author | OLADOSU, Oyindamola | |
| dc.contributor.author | THIERRY, Eloïse | |
| dc.contributor.author | LEON, Oscar | |
| dc.contributor.author | LAVIGNE, Marc | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | ANDREOLA, Marie-Line | |
| dc.contributor.author | DELELIS, Olivier | |
| dc.contributor.author | IVICS, Zoltán | |
| dc.contributor.author | RUFF, Marc | |
| dc.contributor.author | GOUET, Patrice | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | PARISSI, Vincent | |
| dc.date.accessioned | 2023-06-28T10:57:59Z | |
| dc.date.available | 2023-06-28T10:57:59Z | |
| dc.date.issued | 2017 | |
| dc.identifier.issn | 1742-4690 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/183222 | |
| dc.description.abstractEn | Background: Stable insertion of the retroviral DNA genome into host chromatin requires the functional association between the intasome (integrase•viral DNA complex) and the nucleosome. The data from the literature suggest that direct protein-protein contacts between integrase and histones may be involved in anchoring the intasome to the nucleosome. Since histone tails are candidates for interactions with the incoming intasomes we have investigated whether they could participate in modulating the nucleosomal integration process.Results: We show here that histone tails are required for an optimal association between HIV-1 integrase (IN) and the nucleosome for efficient integration. We also demonstrate direct interactions between IN and the amino-terminal tail of human histone H4 in vitro. Structure/function studies enabled us to identify amino acids in the carboxy-terminal domain of IN that are important for this interaction. Analysis of the nucleosome-binding properties of catalytically active mutated INs confirmed that their ability to engage the nucleosome for integration in vitro was affected. Pseudovirus particles bearing mutations that affect the IN/H4 association also showed impaired replication capacity due to altered integration and re-targeting of their insertion sites toward dynamic regions of the chromatin with lower nucleosome occupancy.Conclusions: Collectively, our data support a functional association between HIV-1 IN and histone tails that promotes anchoring of the intasome to nucleosomes and optimal integration into chromatin. | |
| dc.description.sponsorship | Contrôle cellulaire de la sélectivité de l'intégration rétrovirale | en_US |
| dc.description.sponsorship | Infrastructure Française pour la Biologie Structurale Intégrée | en_US |
| dc.language.iso | EN | en_US |
| dc.rights.uri | http://creativecommons.org/licenses/by/ | |
| dc.subject.en | Retroviral integration | |
| dc.subject.en | HIV-1 | |
| dc.subject.en | Integrase | |
| dc.subject.en | Chromatine | |
| dc.subject.en | Nucleosome | |
| dc.subject.en | Histone tails | |
| dc.title.en | Modulation of the functional association between the HIV-1 intasome and the nucleosome by histone amino-terminal tails | |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1186/s12977-017-0378-x | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie | en_US |
| dc.description.sponsorshipEurope | Support to Reinforce the European Strategy Forum for Research Infrastructures | en_US |
| bordeaux.journal | Retrovirology | en_US |
| bordeaux.page | 54 | en_US |
| bordeaux.volume | 14 | en_US |
| bordeaux.hal.laboratories | MFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234 | en_US |
| bordeaux.issue | 1 | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.institution | Université de Bordeaux | |
| bordeaux.institution | Bordeaux INP | |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.import.source | hal | |
| hal.identifier | hal-03532360 | |
| hal.version | 1 | |
| hal.export | false | |
| workflow.import.source | hal | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Retrovirology&rft.date=2017&rft.volume=14&rft.issue=1&rft.spage=54&rft.epage=54&rft.eissn=1742-4690&rft.issn=1742-4690&rft.au=BENLEULMI,%20Mohamed&MATYSIAK,%20Julien&ROBERT,%20Xavier&MISKEY,%20Csaba&MAURO,%20Eric&rft.genre=article |
