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Modulation of the functional association between the HIV-1 intasome and the nucleosome by histone amino-terminal tails

dc.rights.licenseopenen_US
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorBENLEULMI, Mohamed
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorMATYSIAK, Julien
dc.contributor.authorROBERT, Xavier
dc.contributor.authorMISKEY, Csaba
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorMAURO, Eric
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorLAPAILLERIE, Delphine
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorLESBATS, Paul
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorCHAIGNEPAIN, Stéphane
dc.contributor.authorHENRIQUEZ, Daniel
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorCALMELS, Christina
dc.contributor.authorOLADOSU, Oyindamola
dc.contributor.authorTHIERRY, Eloïse
dc.contributor.authorLEON, Oscar
dc.contributor.authorLAVIGNE, Marc
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorANDREOLA, Marie-Line
dc.contributor.authorDELELIS, Olivier
dc.contributor.authorIVICS, Zoltán
dc.contributor.authorRUFF, Marc
dc.contributor.authorGOUET, Patrice
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorPARISSI, Vincent
dc.date.accessioned2023-06-28T10:57:59Z
dc.date.available2023-06-28T10:57:59Z
dc.date.issued2017
dc.identifier.issn1742-4690en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/183222
dc.description.abstractEnBackground: Stable insertion of the retroviral DNA genome into host chromatin requires the functional association between the intasome (integrase•viral DNA complex) and the nucleosome. The data from the literature suggest that direct protein-protein contacts between integrase and histones may be involved in anchoring the intasome to the nucleosome. Since histone tails are candidates for interactions with the incoming intasomes we have investigated whether they could participate in modulating the nucleosomal integration process.Results: We show here that histone tails are required for an optimal association between HIV-1 integrase (IN) and the nucleosome for efficient integration. We also demonstrate direct interactions between IN and the amino-terminal tail of human histone H4 in vitro. Structure/function studies enabled us to identify amino acids in the carboxy-terminal domain of IN that are important for this interaction. Analysis of the nucleosome-binding properties of catalytically active mutated INs confirmed that their ability to engage the nucleosome for integration in vitro was affected. Pseudovirus particles bearing mutations that affect the IN/H4 association also showed impaired replication capacity due to altered integration and re-targeting of their insertion sites toward dynamic regions of the chromatin with lower nucleosome occupancy.Conclusions: Collectively, our data support a functional association between HIV-1 IN and histone tails that promotes anchoring of the intasome to nucleosomes and optimal integration into chromatin.
dc.description.sponsorshipContrôle cellulaire de la sélectivité de l'intégration rétroviraleen_US
dc.description.sponsorshipInfrastructure Française pour la Biologie Structurale Intégréeen_US
dc.language.isoENen_US
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subject.enRetroviral integration
dc.subject.enHIV-1
dc.subject.enIntegrase
dc.subject.enChromatine
dc.subject.enNucleosome
dc.subject.enHistone tails
dc.title.enModulation of the functional association between the HIV-1 intasome and the nucleosome by histone amino-terminal tails
dc.typeArticle de revueen_US
dc.identifier.doi10.1186/s12977-017-0378-xen_US
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologieen_US
dc.description.sponsorshipEuropeSupport to Reinforce the European Strategy Forum for Research Infrastructuresen_US
bordeaux.journalRetrovirologyen_US
bordeaux.page54en_US
bordeaux.volume14en_US
bordeaux.hal.laboratoriesMFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234en_US
bordeaux.issue1en_US
bordeaux.institutionCNRSen_US
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionBordeaux INP
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcehal
hal.identifierhal-03532360
hal.version1
hal.exportfalse
workflow.import.sourcehal
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Retrovirology&rft.date=2017&rft.volume=14&rft.issue=1&rft.spage=54&rft.epage=54&rft.eissn=1742-4690&rft.issn=1742-4690&rft.au=BENLEULMI,%20Mohamed&MATYSIAK,%20Julien&ROBERT,%20Xavier&MISKEY,%20Csaba&MAURO,%20Eric&rft.genre=article


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