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dc.rights.licenseopenen_US
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorMHAYA, Amel
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorBÉGU, Dominique
dc.contributor.authorTOUNSI, Slim
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorARPIN, Corinne
IDREF: 093324626
dc.date.accessioned2023-05-31T14:15:28Z
dc.date.available2023-05-31T14:15:28Z
dc.date.issued2020
dc.identifier.issn0066-4804en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/182391
dc.description.abstractEnMultidrug-resistant strains belonging to the Enterobacter cloacae complex (ECC) group, and especially those belonging to clusters C-III, C-IV, and C-VIII, have increasingly emerged as a leading cause of health care-associated infections, with colistin used as one of the last lines of treatment. However, colistin-resistant ECC strains have emerged. The aim of this study was to prove that MgrB, the negative regulator of the PhoP/PhoQ two-component regulatory system, is involved in colistin resistance in ECC of cluster C-VIII, formerly referred to as Enterobacter hor-maechei subsp. steigerwaltii. An in vitro mutant (Eh22-Mut) was selected from a clinical isolate of Eh22. The sequencing analysis of its mgrB gene showed the presence of one nucleotide deletion leading to the formation of a truncated protein of six instead of 47 amino acids. The wild-type mgrB gene from Eh22 and that of a clinical strain of Klebsiella pneumoniae used as controls were cloned, and the corresponding recombinant plasmids were used for complementation assays. The results showed a fully restored susceptibility to colistin and confirmed for the first time that mgrB gene expression plays a key role in acquired resistance to colistin in ECC strains.
dc.language.isoENen_US
dc.subject.enEnterobacter cloacae complex
dc.subject.enEnterobacter hormaechei subsp steigerwaltii
dc.subject.encolistin resistance
dc.subject.enMgrB
dc.subject.enPhoPQ regulatory system
dc.title.enMgrB Inactivation Is Responsible for Acquired Resistance to Colistin in Enterobacter hormaechei subsp. steigerwaltii
dc.typeArticle de revueen_US
dc.identifier.doi10.1128/AAC.00128-20en_US
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie/Bactériologieen_US
dc.subject.halSciences du Vivant [q-bio]/Sciences pharmaceutiques/Pharmacologieen_US
bordeaux.journalAntimicrobial Agents and Chemotherapyen_US
bordeaux.pagee00128-20en_US
bordeaux.volume64en_US
bordeaux.hal.laboratoriesMFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234en_US
bordeaux.issue6en_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcehal
hal.identifierhal-02980500
hal.version1
hal.exportfalse
workflow.import.sourcehal
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Antimicrobial%20Agents%20and%20Chemotherapy&rft.date=2020&rft.volume=64&rft.issue=6&rft.spage=e00128-20&rft.epage=e00128-20&rft.eissn=0066-4804&rft.issn=0066-4804&rft.au=MHAYA,%20Amel&B%C3%89GU,%20Dominique&TOUNSI,%20Slim&ARPIN,%20Corinne&rft.genre=article


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