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MgrB Inactivation Is Responsible for Acquired Resistance to Colistin in Enterobacter hormaechei subsp. steigerwaltii
| dc.rights.license | open | en_US |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | MHAYA, Amel | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | BÉGU, Dominique | |
| dc.contributor.author | TOUNSI, Slim | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | ARPIN, Corinne
IDREF: 093324626 | |
| dc.date.accessioned | 2023-05-31T14:15:28Z | |
| dc.date.available | 2023-05-31T14:15:28Z | |
| dc.date.issued | 2020 | |
| dc.identifier.issn | 0066-4804 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/182391 | |
| dc.description.abstractEn | Multidrug-resistant strains belonging to the Enterobacter cloacae complex (ECC) group, and especially those belonging to clusters C-III, C-IV, and C-VIII, have increasingly emerged as a leading cause of health care-associated infections, with colistin used as one of the last lines of treatment. However, colistin-resistant ECC strains have emerged. The aim of this study was to prove that MgrB, the negative regulator of the PhoP/PhoQ two-component regulatory system, is involved in colistin resistance in ECC of cluster C-VIII, formerly referred to as Enterobacter hor-maechei subsp. steigerwaltii. An in vitro mutant (Eh22-Mut) was selected from a clinical isolate of Eh22. The sequencing analysis of its mgrB gene showed the presence of one nucleotide deletion leading to the formation of a truncated protein of six instead of 47 amino acids. The wild-type mgrB gene from Eh22 and that of a clinical strain of Klebsiella pneumoniae used as controls were cloned, and the corresponding recombinant plasmids were used for complementation assays. The results showed a fully restored susceptibility to colistin and confirmed for the first time that mgrB gene expression plays a key role in acquired resistance to colistin in ECC strains. | |
| dc.language.iso | EN | en_US |
| dc.subject.en | Enterobacter cloacae complex | |
| dc.subject.en | Enterobacter hormaechei subsp steigerwaltii | |
| dc.subject.en | colistin resistance | |
| dc.subject.en | MgrB | |
| dc.subject.en | PhoPQ regulatory system | |
| dc.title.en | MgrB Inactivation Is Responsible for Acquired Resistance to Colistin in Enterobacter hormaechei subsp. steigerwaltii | |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1128/AAC.00128-20 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Bactériologie | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Sciences pharmaceutiques/Pharmacologie | en_US |
| bordeaux.journal | Antimicrobial Agents and Chemotherapy | en_US |
| bordeaux.page | e00128-20 | en_US |
| bordeaux.volume | 64 | en_US |
| bordeaux.hal.laboratories | MFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234 | en_US |
| bordeaux.issue | 6 | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.import.source | hal | |
| hal.identifier | hal-02980500 | |
| hal.version | 1 | |
| hal.export | false | |
| workflow.import.source | hal | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Antimicrobial%20Agents%20and%20Chemotherapy&rft.date=2020&rft.volume=64&rft.issue=6&rft.spage=e00128-20&rft.epage=e00128-20&rft.eissn=0066-4804&rft.issn=0066-4804&rft.au=MHAYA,%20Amel&B%C3%89GU,%20Dominique&TOUNSI,%20Slim&ARPIN,%20Corinne&rft.genre=article |
