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dc.rights.licenseopenen_US
dc.contributor.authorFERREIRA, William T.
dc.contributor.authorHONG, Huynh A.
dc.contributor.authorADAMS, James R. G.
dc.contributor.authorHESS, Mateusz
dc.contributor.authorKOTOWICZ, Natalia K.
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorTAN, Sisareuth
dc.contributor.authorFERRARI, Enrico
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorBRISSON, Alain
dc.contributor.authorZENTEK, Jurgen
dc.contributor.authorSOLOVIEV, Mikhail
dc.contributor.authorCUTTING, Simon M.
dc.date.accessioned2023-05-22T15:33:18Z
dc.date.available2023-05-22T15:33:18Z
dc.date.issued2022-04-19
dc.identifier.issn2227-9059en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/182244
dc.description.abstractEnClostridioides difficile is an environmentally acquired, anaerobic, spore-forming bacterium which ordinarily causes disease following antibiotic-mediated dysbiosis of the intestinal microbiota. Although much is understood regarding the life cycle of C. difficile, the fate of C. difficile spores upon ingestion remains unclear, and the underlying factors that predispose an individual to colonization and subsequent development of C. difficile infection (CDI) are not fully understood. Here, we show that Bacillus, a ubiquitous and environmentally acquired, spore-forming bacterium is associated with colonization resistance to C. difficile. Using animal models, we first provide evidence that animals housed under conditions that mimic reduced environmental exposure have an increased susceptibility to CDI, correlating with a loss in Bacillus. Lipopeptide micelles (~10 nm) produced by some Bacilli isolated from the gastro-intestinal (GI)-tract and shown to have potent inhibitory activity to C. difficile have recently been reported. We show here that these micelles, that we refer to as heterogenous lipopeptide lytic micelles (HELMs), act synergistically with components present in the small intestine to augment inhibitory activity against C. difficile. Finally, we show that provision of HELM-producing Bacillus to microbiota-depleted animals suppresses C. difficile colonization thereby demonstrating the significant role played by Bacillus in colonization resistance. In the wider context, our study further demonstrates the importance of environmental microbes on susceptibility to pathogen colonization.
dc.language.isoENen_US
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.subject.enClostridioides difficile infection
dc.subject.enBacillus
dc.subject.enEnvironmental bacteria
dc.subject.enAllochthonous bacteria
dc.subject.enLipopeptides
dc.title.enEnvironmentally Acquired Bacillus and Their Role in C. difficile Colonization Resistance
dc.typeArticle de revueen_US
dc.identifier.doi10.3390/biomedicines10050930en_US
dc.subject.halChimie/Matériauxen_US
bordeaux.journalBiomedicinesen_US
bordeaux.volume10en_US
bordeaux.hal.laboratoriesCBMN : Chimie & de Biologie des Membranes & des Nano-objets - UMR 5248en_US
bordeaux.issue5en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-04102862
hal.version1
hal.date.transferred2023-05-22T15:33:33Z
hal.exporttrue
dc.rights.ccCC BYen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biomedicines&rft.date=2022-04-19&rft.volume=10&rft.issue=5&rft.eissn=2227-9059&rft.issn=2227-9059&rft.au=FERREIRA,%20William%20T.&HONG,%20Huynh%20A.&ADAMS,%20James%20R.%20G.&HESS,%20Mateusz&KOTOWICZ,%20Natalia%20K.&rft.genre=article


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