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hal.structure.identifierCentre de Recherche Paul Pascal [CRPP]
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorOLDENBURG, Simone
hal.structure.identifierCentre de Recherche Paul Pascal [CRPP]
dc.contributor.authorBUISSON, Lionel
hal.structure.identifierCentre de Recherche Paul Pascal [CRPP]
dc.contributor.authorBENEYTON, Thomas
hal.structure.identifierCentre de Recherche Paul Pascal [CRPP]
dc.contributor.authorPEKIN, Deniz
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorTHONNUS, Magali
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorBRINGAUD, Frédéric
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorRIVIÈRE, Loïc
hal.structure.identifierCentre de Recherche Paul Pascal [CRPP]
hal.structure.identifierInstitut universitaire de France [IUF]
dc.contributor.authorBARET, Jean-Christophe
IDREF: 19204690X
dc.date.accessioned2023-05-22T10:58:42Z
dc.date.available2023-05-22T10:58:42Z
dc.date.issued2021-09-14
dc.identifier.issn2045-2322
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/182223
dc.description.abstractEnTrypanosome parasites are infecting mammals in Sub-Saharan Africa and are transmitted between hosts through bites of the tsetse fly. The transmission from the insect vector to the mammal host causes a number of metabolic and physiological changes. A fraction of the population continuously adapt to the immune system of the host, indicating heterogeneity at the population level. Yet, the cell to cell variability in populations is mostly unknown. We develop here an analytical method for quantitative measurements at the single cell level based on encapsulation and cultivation of single-cell Trypanosoma brucei in emulsion droplets. We first show that mammalian stage trypanosomes survive for several hours to days in droplets, with an influence of droplet size on both survival and growth. We unravel various growth patterns within a population and find that droplet cultivation of trypanosomes results in 10-fold higher cell densities of the highest dividing cell variants compared to standard cultivation techniques. Some variants reach final cell titers in droplets closer to what is observed in nature than standard culture, of practical interest for cell production. Droplet microfluidics is therefore a promising tool for trypanosome cultivation and analysis with further potential for high-throughput single cell trypanosome analysis.
dc.language.isoen
dc.publisherNature Publishing Group
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.title.enConfining Trypanosoma brucei in emulsion droplets reveals population variabilities in division rates and improves in vitro cultivation
dc.typeArticle de revueen_US
dc.identifier.doi10.1038/s41598-021-97356-7
dc.subject.halSciences du Vivant [q-bio]/Biologie cellulaire
dc.subject.halChimie/Chimie analytique
bordeaux.journalScientific Reportsen_US
bordeaux.volume11
bordeaux.hal.laboratoriesMFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234en_US
bordeaux.issue1
bordeaux.institutionCNRS
bordeaux.peerReviewedoui
bordeaux.import.sourcehal
hal.identifierhal-03347077
hal.version1
hal.exportfalse
workflow.import.sourcehal
dc.rights.ccCC BYen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Scientific%20Reports&rft.date=2021-09-14&rft.volume=11&rft.issue=1&rft.eissn=2045-2322&rft.issn=2045-2322&rft.au=OLDENBURG,%20Simone&BUISSON,%20Lionel&BENEYTON,%20Thomas&PEKIN,%20Deniz&THONNUS,%20Magali&rft.genre=article


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