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Modulation of the intrinsic chromatin binding property of HIV-1 integrase by LEDGF/p75

dc.rights.licenseopenen_US
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
hal.structure.identifierSFR TransBioMed [Bordeaux]
dc.contributor.authorLAPAILLERIE, Delphine
dc.contributor.authorLELANDAIS, Benoît
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorMAURO, Eric
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorLAGADEC, Floriane
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorTUMIOTTO, Camille
hal.structure.identifierPaul-Ehrlich-Institute - Federal Institute for Vaccines and Biomedicines [EPI]
dc.contributor.authorMISKEY, Csaba
hal.structure.identifierCentre Hospitalier Universitaire de Bordeaux [CHU Bordeaux]
dc.contributor.authorFERRAN, Guillaume
hal.structure.identifierCentre Hospitalier Universitaire de Bordeaux [CHU Bordeaux]
dc.contributor.authorKUSCHNER, Natacha
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorCALMELS, Christina
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorMÉTIFIOT, Mathieu
hal.structure.identifierCentre Hospitalier Universitaire de Bordeaux [CHU Bordeaux]
dc.contributor.authorROORYCK, Caroline
hal.structure.identifierPaul-Ehrlich-Institute - Federal Institute for Vaccines and Biomedicines [EPI]
dc.contributor.authorIVICS, Zoltan
hal.structure.identifierInstitut de Génétique et de Biologie Moléculaire et Cellulaire [IGBMC]
dc.contributor.authorRUFF, Marc
dc.contributor.authorZIMMER, Christophe
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorLESBATS, Paul
hal.structure.identifierCentre Hospitalier Universitaire de Bordeaux [CHU Bordeaux]
dc.contributor.authorTOUTAIN, Jérôme
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorPARISSI, Vincent
dc.date.accessioned2023-05-22T10:31:11Z
dc.date.available2023-05-22T10:31:11Z
dc.date.issued2021-11-08
dc.identifier.issn0305-1048en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/182220
dc.description.abstractEnAbstract The stable insertion of the retroviral genome into the host chromosomes requires the association between integration complexes and cellular chromatin via the interaction between retroviral integrase and the nucleosomal target DNA. This final association may involve the chromatin-binding properties of both the retroviral integrase and its cellular cofactor LEDGF/p75. To investigate this and better understand the LEDGF/p75-mediated chromatin tethering of HIV-1 integrase, we used a combination of biochemical and chromosome-binding assays. Our study revealed that retroviral integrase has an intrinsic ability to bind and recognize specific chromatin regions in metaphase even in the absence of its cofactor. Furthermore, this integrase chromatin-binding property was modulated by the interaction with its cofactor LEDGF/p75, which redirected the enzyme to alternative chromosome regions. We also better determined the chromatin features recognized by each partner alone or within the functional intasome, as well as the chronology of efficient LEDGF/p75-mediated targeting of HIV-1 integrase to chromatin. Our data support a new chromatin-binding function of integrase acting in concert with LEDGF/p75 for the optimal association with the nucleosomal substrate. This work also provides additional information about the behavior of retroviral integration complexes in metaphase chromatin and the mechanism of action of LEDGF/p75 in this specific context.
dc.language.isoENen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc/
dc.title.enModulation of the intrinsic chromatin binding property of HIV-1 integrase by LEDGF/p75
dc.typeArticle de revueen_US
dc.identifier.doi10.1093/nar/gkab886en_US
dc.subject.halSciences du Vivant [q-bio]en_US
bordeaux.journalNucleic Acids Researchen_US
bordeaux.page11241-11256en_US
bordeaux.volume49en_US
bordeaux.hal.laboratoriesMFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234en_US
bordeaux.issue19en_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcehal
hal.identifierhal-03795503
hal.version1
hal.exportfalse
workflow.import.sourcehal
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Nucleic%20Acids%20Research&rft.date=2021-11-08&rft.volume=49&rft.issue=19&rft.spage=11241-11256&rft.epage=11241-11256&rft.eissn=0305-1048&rft.issn=0305-1048&rft.au=LAPAILLERIE,%20Delphine&LELANDAIS,%20Beno%C3%AEt&MAURO,%20Eric&LAGADEC,%20Floriane&TUMIOTTO,%20Camille&rft.genre=article


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