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hal.structure.identifierMicroenvironment, Cell Differentiation, Immunology and Cancer [MICMAC]
hal.structure.identifierEtablissement français du sang [Rennes] [EFS Bretagne]
dc.contributor.authorLAMAISON, Claire
hal.structure.identifierUniversity of Toronto
dc.contributor.authorLATOUR, Simon
hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
dc.contributor.authorHÉLAINE, Nelson
hal.structure.identifierActions for OnCogenesis understanding and Target Identification in ONcology [ACTION]
dc.contributor.authorLE MORVAN, Valerie
hal.structure.identifierMicroenvironment, Cell Differentiation, Immunology and Cancer [MICMAC]
hal.structure.identifierEtablissement français du sang [Rennes] [EFS Bretagne]
dc.contributor.authorSAINT-VANNE, Julien
hal.structure.identifierActions for OnCogenesis understanding and Target Identification in ONcology [ACTION]
dc.contributor.authorMAHOUCHE, Isabelle
hal.structure.identifierMicroenvironment, Cell Differentiation, Immunology and Cancer [MICMAC]
dc.contributor.authorMONVOISIN, Celine
hal.structure.identifierActions for OnCogenesis understanding and Target Identification in ONcology [ACTION]
dc.contributor.authorDUSSERT, Christelle
hal.structure.identifierUniversité de Bordeaux [UB]
dc.contributor.authorANDRIQUE, Laëtitia
hal.structure.identifierMicroenvironment, Cell Differentiation, Immunology and Cancer [MICMAC]
hal.structure.identifierEtablissement français du sang [Rennes] [EFS Bretagne]
dc.contributor.authorDELEURME, Laurent
hal.structure.identifierMicroenvironment, Cell Differentiation, Immunology and Cancer [MICMAC]
dc.contributor.authorDESSAUGE, Elise
hal.structure.identifierUniversité de Rennes [UR]
dc.contributor.authorPANGAULT, Celine
hal.structure.identifierInstitut Curie [Paris]
dc.contributor.authorBAULANDE, Sylvain
hal.structure.identifierInstitut Curie [Paris]
hal.structure.identifierPlateforme de sequencage ICGEX
dc.contributor.authorLEGOIX, Patricia
hal.structure.identifierUniversité de Rennes [UR]
dc.contributor.authorSEFFALS, Marine
hal.structure.identifierActions for OnCogenesis understanding and Target Identification in ONcology [ACTION]
dc.contributor.authorBROCA-BRISSON, Lea
hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
dc.contributor.authorALESSANDRI, Kévin
hal.structure.identifierBordeaux Research In Translational Oncology [Bordeaux] [BaRITOn]
dc.contributor.authorPROCHAZKOVA-CARLOTTI, Martina
hal.structure.identifierInstitut Bergonié [Bordeaux]
dc.contributor.authorSOUBEYRAN, Pierre
hal.structure.identifierCHU Bordeaux
dc.contributor.authorMERLIO, Jean-Philippe
hal.structure.identifierMicroenvironment, Cell Differentiation, Immunology and Cancer [MICMAC]
hal.structure.identifierEtablissement français du sang [Rennes] [EFS Bretagne]
dc.contributor.authorMOURCIN, Frédéric
hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
dc.contributor.authorNASSOY, Pierre
hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
dc.contributor.authorRECHER, Gaëlle
hal.structure.identifierMicroenvironment, Cell Differentiation, Immunology and Cancer [MICMAC]
hal.structure.identifierEtablissement français du sang [Rennes] [EFS Bretagne]
dc.contributor.authorTARTE, Karin
hal.structure.identifierBordeaux Research In Translational Oncology [Bordeaux] [BaRITOn]
dc.contributor.authorBRESSON-BEPOLDIN, Laurence
dc.date.accessioned2023-05-12T10:34:37Z
dc.date.available2023-05-12T10:34:37Z
dc.date.issued2021-09-23
dc.identifier.issn2473-9529
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/181478
dc.description.abstractEnNon-Hodgkin B-cell lymphomas (B-NHL) mainly develop within lymph nodes (LN) as densely packed aggregates of tumor cells and their surrounding microenvironment, creating a tumor niche specific to each lymphoma subtypes. In vitro preclinical models mimicking biomechanical forces, cellular microenvironment, and 3D organization of B-cell lymphomas remain scarce, while all these parameters constitute key determinants of lymphomagenesis and drug resistance. Using a microfluidic method based on cell encapsulation inside permeable, elastic, and hollow alginate microspheres, we developed a new tunable 3D-model incorporating lymphoma B cells, extracellular matrix (ECM), and/or tonsil stromal cells (TSC). We revealed that under 3D confinement lymphoma B cells were able to form cohesive spheroids resulting from overexpression of ECM components. Moreover, lymphoma B cells and TSC dynamically formed self-organized 3D spheroids favoring spheroid growth. 3D culture induced resistance to classical chemotherapeutic agent doxorubicin, but not to BCL2 inhibitor ABT-199, identifying this approach as a relevant in vitro model to assess the activity of therapeutic agents in B-NHL. RNAseq analysis highlighted the synergy of 3D, ECM, and TSC in upregulating similar pathways in malignant B cells in vitro than those overexpressed in primary lymphoma cells in situ. Finally, our 3D model including ECM and TSC allowed long-term in vitro survival of primary follicular lymphoma B cells. In conclusion, we propose a new high throughput 3D model mimicking lymphoma tumor niche and making it possible to study the dynamic relationship between lymphoma B cells and their microenvironment and to screen new anti-cancer drugs.
dc.description.sponsorshipDéveloppement d'une Plateforme Nationale pour la médecine régénératrice
dc.description.sponsorshipDéveloppment d'une infrastructure française distribuée coordonnée - ANR-10-INBS-0004
dc.description.sponsorshipEquipement de biologie intégrative du cancer pour une médecine personnalisée
dc.language.isoen
dc.publisherThe American Society of Hematology
dc.rights.urihttp://creativecommons.org/licenses/by-nc/
dc.subject.enalginates
dc.subject.enb-lymphocytes
dc.subject.enlymphoma
dc.subject.enb-cell lymphomas
dc.subject.enneoplasms
dc.subject.enantineoplastic agents
dc.subject.enbcl-2 protein
dc.subject.encancer
dc.subject.endoxorubicin
dc.subject.enfollicular lymphoma
dc.title.enA novel 3D culture model recapitulates primary FL B cell features and promotes their survival
dc.typeArticle de revue
dc.identifier.doi10.1182/bloodadvances.2020003949
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Cancer
bordeaux.journalBlood Advances
bordeaux.hal.laboratoriesLaboratoire Photonique, Numérique et Nanosciences (LP2N) - UMR 5298*
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionCNRS
bordeaux.peerReviewedoui
hal.identifierhal-03367891
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03367891v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Blood%20Advances&rft.date=2021-09-23&rft.eissn=2473-9529&rft.issn=2473-9529&rft.au=LAMAISON,%20Claire&LATOUR,%20Simon&H%C3%89LAINE,%20Nelson&LE%20MORVAN,%20Valerie&SAINT-VANNE,%20Julien&rft.genre=article


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