A novel 3D culture model recapitulates primary FL B cell features and promotes their survival
LAMAISON, Claire
Microenvironment, Cell Differentiation, Immunology and Cancer [MICMAC]
Etablissement français du sang [Rennes] [EFS Bretagne]
Leer más >
Microenvironment, Cell Differentiation, Immunology and Cancer [MICMAC]
Etablissement français du sang [Rennes] [EFS Bretagne]
LAMAISON, Claire
Microenvironment, Cell Differentiation, Immunology and Cancer [MICMAC]
Etablissement français du sang [Rennes] [EFS Bretagne]
Microenvironment, Cell Differentiation, Immunology and Cancer [MICMAC]
Etablissement français du sang [Rennes] [EFS Bretagne]
LE MORVAN, Valerie
Actions for OnCogenesis understanding and Target Identification in ONcology [ACTION]
Actions for OnCogenesis understanding and Target Identification in ONcology [ACTION]
SAINT-VANNE, Julien
Microenvironment, Cell Differentiation, Immunology and Cancer [MICMAC]
Etablissement français du sang [Rennes] [EFS Bretagne]
Microenvironment, Cell Differentiation, Immunology and Cancer [MICMAC]
Etablissement français du sang [Rennes] [EFS Bretagne]
MAHOUCHE, Isabelle
Actions for OnCogenesis understanding and Target Identification in ONcology [ACTION]
Actions for OnCogenesis understanding and Target Identification in ONcology [ACTION]
DUSSERT, Christelle
Actions for OnCogenesis understanding and Target Identification in ONcology [ACTION]
Actions for OnCogenesis understanding and Target Identification in ONcology [ACTION]
DELEURME, Laurent
Microenvironment, Cell Differentiation, Immunology and Cancer [MICMAC]
Etablissement français du sang [Rennes] [EFS Bretagne]
Microenvironment, Cell Differentiation, Immunology and Cancer [MICMAC]
Etablissement français du sang [Rennes] [EFS Bretagne]
BROCA-BRISSON, Lea
Actions for OnCogenesis understanding and Target Identification in ONcology [ACTION]
Actions for OnCogenesis understanding and Target Identification in ONcology [ACTION]
MOURCIN, Frédéric
Microenvironment, Cell Differentiation, Immunology and Cancer [MICMAC]
Etablissement français du sang [Rennes] [EFS Bretagne]
Microenvironment, Cell Differentiation, Immunology and Cancer [MICMAC]
Etablissement français du sang [Rennes] [EFS Bretagne]
TARTE, Karin
Microenvironment, Cell Differentiation, Immunology and Cancer [MICMAC]
Etablissement français du sang [Rennes] [EFS Bretagne]
< Leer menos
Microenvironment, Cell Differentiation, Immunology and Cancer [MICMAC]
Etablissement français du sang [Rennes] [EFS Bretagne]
Idioma
en
Article de revue
Este ítem está publicado en
Blood Advances. 2021-09-23
The American Society of Hematology
Resumen en inglés
Non-Hodgkin B-cell lymphomas (B-NHL) mainly develop within lymph nodes (LN) as densely packed aggregates of tumor cells and their surrounding microenvironment, creating a tumor niche specific to each lymphoma subtypes. In ...Leer más >
Non-Hodgkin B-cell lymphomas (B-NHL) mainly develop within lymph nodes (LN) as densely packed aggregates of tumor cells and their surrounding microenvironment, creating a tumor niche specific to each lymphoma subtypes. In vitro preclinical models mimicking biomechanical forces, cellular microenvironment, and 3D organization of B-cell lymphomas remain scarce, while all these parameters constitute key determinants of lymphomagenesis and drug resistance. Using a microfluidic method based on cell encapsulation inside permeable, elastic, and hollow alginate microspheres, we developed a new tunable 3D-model incorporating lymphoma B cells, extracellular matrix (ECM), and/or tonsil stromal cells (TSC). We revealed that under 3D confinement lymphoma B cells were able to form cohesive spheroids resulting from overexpression of ECM components. Moreover, lymphoma B cells and TSC dynamically formed self-organized 3D spheroids favoring spheroid growth. 3D culture induced resistance to classical chemotherapeutic agent doxorubicin, but not to BCL2 inhibitor ABT-199, identifying this approach as a relevant in vitro model to assess the activity of therapeutic agents in B-NHL. RNAseq analysis highlighted the synergy of 3D, ECM, and TSC in upregulating similar pathways in malignant B cells in vitro than those overexpressed in primary lymphoma cells in situ. Finally, our 3D model including ECM and TSC allowed long-term in vitro survival of primary follicular lymphoma B cells. In conclusion, we propose a new high throughput 3D model mimicking lymphoma tumor niche and making it possible to study the dynamic relationship between lymphoma B cells and their microenvironment and to screen new anti-cancer drugs.< Leer menos
Palabras clave en inglés
alginates
b-lymphocytes
lymphoma
b-cell lymphomas
neoplasms
antineoplastic agents
bcl-2 protein
cancer
doxorubicin
follicular lymphoma
Proyecto ANR
Développement d'une Plateforme Nationale pour la médecine régénératrice
Développment d'une infrastructure française distribuée coordonnée - ANR-10-INBS-0004
Equipement de biologie intégrative du cancer pour une médecine personnalisée
Développment d'une infrastructure française distribuée coordonnée - ANR-10-INBS-0004
Equipement de biologie intégrative du cancer pour une médecine personnalisée
Orígen
Importado de HalCentros de investigación