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Performance of real-time PCR in suspected haemodialysis catheter-related bloodstream infection: a proof-of-concept study.
| dc.rights.license | open | en_US |
| hal.structure.identifier | CHU de Bordeaux Pellegrin [Bordeaux] | |
| dc.contributor.author | ACQUIER, Mathieu | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | ZABALA, Arnaud | |
| hal.structure.identifier | CHU de Bordeaux Pellegrin [Bordeaux] | |
| dc.contributor.author | DE PRÉCIGOUT, Valérie | |
| hal.structure.identifier | CHU de Bordeaux Pellegrin [Bordeaux] | |
| dc.contributor.author | DELMAS, Yahsou | |
| hal.structure.identifier | CHU de Bordeaux Pellegrin [Bordeaux] | |
| dc.contributor.author | DUBOIS, Véronique | |
| hal.structure.identifier | CHU de Bordeaux Pellegrin [Bordeaux] | |
| dc.contributor.author | DE LA FAILLE, Renaud | |
| hal.structure.identifier | Biologie des maladies cardiovasculaires = Biology of Cardiovascular Diseases | |
| dc.contributor.author | RUBIN, Sébastien | |
| hal.structure.identifier | Bioingénierie tissulaire [BIOTIS] | |
| dc.contributor.author | COMBE, Christian
ORCID: 0000-0002-0360-573X IDREF: 58708871 | |
| hal.structure.identifier | CHU de Bordeaux Pellegrin [Bordeaux] | |
| dc.contributor.author | M'ZALI, Fatima | |
| hal.structure.identifier | Immunology from Concept and Experiments to Translation = Immunologie Conceptuelle, Expérimentale et Translationnelle [ImmunoConcept] | |
| dc.contributor.author | KAMINSKI, Hannah | |
| dc.date.accessioned | 2023-05-11T12:58:39Z | |
| dc.date.available | 2023-05-11T12:58:39Z | |
| dc.date.issued | 2023-03-01 | |
| dc.identifier.issn | 2048-8505 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/180990 | |
| dc.description.abstractEn | Catheter-related bloodstream infections (CRBIs) remain a major cause of mortality in haemodialysis (HD) patients with central venous catheters (CVCs), especially because of the non-specific symptomatology and the delay in microbiological diagnosis with possible use of non-optimal empiric antibiotics. Moreover, empiric broad-spectrum antibiotics increase antibiotic resistance development. This study aims to evaluate the diagnostic performance of real-time polymerase chain reaction (rt-PCR) in suspected HD CRBIs compared with blood cultures. A blood sample for rt-PCR was collected simultaneously with each pair of blood cultures for suspected HD CRBI. The rt-PCR was performed on the whole blood, without any enrichment stage and with specific DNA primers: 16S (universal bacterial), spp., and . Each successive patient with a suspected HD CRBI in the HD centre of Bordeaux University Hospital was included. Performance tests were used to compare the result obtained in each rt-PCR assay with its corresponding routine blood culture. Eighty-four paired samples were collected and compared for 40 suspected HD CRBI events in 37 patients. Among these, 13 (32.5%) were diagnosed as HD CRBI. All rt-PCRs except (insufficient number of positive samples) showed high diagnostic performances within 3.5 h: 16S (sensitivity 100%, specificity 78%), spp. (sensitivity 100%, specificity 97%), (sensitivity 100%, specificity 99%). Based on the rt-PCR results, antibiotics could be more appropriately targeted, thus cutting anti-cocci Gram-positive therapy from 77% to 29%. The performance of rt-PCR in suspected HD CRBI events showed fast and high diagnostic accuracy. Its use would improve HD CRBI management with an antibiotic consumption decrease. | |
| dc.language.iso | EN | en_US |
| dc.rights | Attribution 3.0 United States | * |
| dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | * |
| dc.subject | Article clinique | |
| dc.title.en | Performance of real-time PCR in suspected haemodialysis catheter-related bloodstream infection: a proof-of-concept study. | |
| dc.title.alternative | Clin Kidney J | en_US |
| dc.type | Article de revue | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Médecine humaine et pathologie | en_US |
| dc.identifier.pubmed | 36865002 | en_US |
| bordeaux.journal | Clinical Kidney Journal | en_US |
| bordeaux.page | 494-500 | en_US |
| bordeaux.volume | 16 | en_US |
| bordeaux.hal.laboratories | Biologie des maladies cardiovasculaires (BMC) - UMR 1034 | en_US |
| bordeaux.issue | 3 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | INSERM | en_US |
| bordeaux.institution | CNRS | |
| bordeaux.institution | CHU de Bordeaux | |
| bordeaux.institution | Institut Bergonié | |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.import.source | pubmed | |
| hal.identifier | hal-04095065 | |
| hal.version | 1 | |
| hal.date.transferred | 2023-05-11T12:58:43Z | |
| hal.export | true | |
| workflow.import.source | pubmed | |
| dc.rights.cc | CC BY | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Clinical%20Kidney%20Journal&rft.date=2023-03-01&rft.volume=16&rft.issue=3&rft.spage=494-500&rft.epage=494-500&rft.eissn=2048-8505&rft.issn=2048-8505&rft.au=ACQUIER,%20Mathieu&ZABALA,%20Arnaud&DE%20PR%C3%89CIGOUT,%20Val%C3%A9rie&DELMAS,%20Yahsou&DUBOIS,%20V%C3%A9ronique&rft.genre=article |
