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dc.rights.licenseopenen_US
hal.structure.identifierCHU de Bordeaux Pellegrin [Bordeaux]
dc.contributor.authorACQUIER, Mathieu
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorZABALA, Arnaud
hal.structure.identifierCHU de Bordeaux Pellegrin [Bordeaux]
dc.contributor.authorDE PRÉCIGOUT, Valérie
hal.structure.identifierCHU de Bordeaux Pellegrin [Bordeaux]
dc.contributor.authorDELMAS, Yahsou
hal.structure.identifierCHU de Bordeaux Pellegrin [Bordeaux]
dc.contributor.authorDUBOIS, Véronique
hal.structure.identifierCHU de Bordeaux Pellegrin [Bordeaux]
dc.contributor.authorDE LA FAILLE, Renaud
hal.structure.identifierBiologie des maladies cardiovasculaires = Biology of Cardiovascular Diseases
dc.contributor.authorRUBIN, Sébastien
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorCOMBE, Christian
ORCID: 0000-0002-0360-573X
IDREF: 58708871
hal.structure.identifierCHU de Bordeaux Pellegrin [Bordeaux]
dc.contributor.authorM'ZALI, Fatima
hal.structure.identifierImmunology from Concept and Experiments to Translation = Immunologie Conceptuelle, Expérimentale et Translationnelle [ImmunoConcept]
dc.contributor.authorKAMINSKI, Hannah
dc.date.accessioned2023-05-11T12:58:39Z
dc.date.available2023-05-11T12:58:39Z
dc.date.issued2023-03-01
dc.identifier.issn2048-8505en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/180990
dc.description.abstractEnCatheter-related bloodstream infections (CRBIs) remain a major cause of mortality in haemodialysis (HD) patients with central venous catheters (CVCs), especially because of the non-specific symptomatology and the delay in microbiological diagnosis with possible use of non-optimal empiric antibiotics. Moreover, empiric broad-spectrum antibiotics increase antibiotic resistance development. This study aims to evaluate the diagnostic performance of real-time polymerase chain reaction (rt-PCR) in suspected HD CRBIs compared with blood cultures. A blood sample for rt-PCR was collected simultaneously with each pair of blood cultures for suspected HD CRBI. The rt-PCR was performed on the whole blood, without any enrichment stage and with specific DNA primers: 16S (universal bacterial), spp., and . Each successive patient with a suspected HD CRBI in the HD centre of Bordeaux University Hospital was included. Performance tests were used to compare the result obtained in each rt-PCR assay with its corresponding routine blood culture. Eighty-four paired samples were collected and compared for 40 suspected HD CRBI events in 37 patients. Among these, 13 (32.5%) were diagnosed as HD CRBI. All rt-PCRs except (insufficient number of positive samples) showed high diagnostic performances within 3.5 h: 16S (sensitivity 100%, specificity 78%), spp. (sensitivity 100%, specificity 97%), (sensitivity 100%, specificity 99%). Based on the rt-PCR results, antibiotics could be more appropriately targeted, thus cutting anti-cocci Gram-positive therapy from 77% to 29%. The performance of rt-PCR in suspected HD CRBI events showed fast and high diagnostic accuracy. Its use would improve HD CRBI management with an antibiotic consumption decrease.
dc.language.isoENen_US
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.subjectArticle clinique
dc.title.enPerformance of real-time PCR in suspected haemodialysis catheter-related bloodstream infection: a proof-of-concept study.
dc.title.alternativeClin Kidney Jen_US
dc.typeArticle de revueen_US
dc.subject.halSciences du Vivant [q-bio]/Médecine humaine et pathologieen_US
dc.identifier.pubmed36865002en_US
bordeaux.journalClinical Kidney Journalen_US
bordeaux.page494-500en_US
bordeaux.volume16en_US
bordeaux.hal.laboratoriesBiologie des maladies cardiovasculaires (BMC) - UMR 1034en_US
bordeaux.issue3en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionINSERMen_US
bordeaux.institutionCNRS
bordeaux.institutionCHU de Bordeaux
bordeaux.institutionInstitut Bergonié
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcepubmed
hal.identifierhal-04095065
hal.version1
hal.date.transferred2023-05-11T12:58:43Z
hal.exporttrue
workflow.import.sourcepubmed
dc.rights.ccCC BYen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Clinical%20Kidney%20Journal&rft.date=2023-03-01&rft.volume=16&rft.issue=3&rft.spage=494-500&rft.epage=494-500&rft.eissn=2048-8505&rft.issn=2048-8505&rft.au=ACQUIER,%20Mathieu&ZABALA,%20Arnaud&DE%20PR%C3%89CIGOUT,%20Val%C3%A9rie&DELMAS,%20Yahsou&DUBOIS,%20V%C3%A9ronique&rft.genre=article


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